RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential

Global agricultural production faces unprecedented challenges due to climate crisis, biodiversity loss, and increasing population pressure, while there is a growing demand for sustainable and eco-conscious food production systems. Traditional methods of crop protection like the use of synthetic chemical pesticides are becoming less effective due to the high resistance development in major insect pests. Moreover, their overuse has raised numerous environmental concerns. In this context, RNA interference (RNAi) has emerged as a promising and environmentally friendly alternative to traditional pesticides, with a more sustainable way of managing pests. This review systematically identifies promising RNAi target gene families for insect pest control, particularly key developmental genes. The selected genes were chosen based on demonstrated RNAi efficacy in at least three different insect species, emphasizing their broad applicability and potential impact. It also discusses the translation of RNAi technologies from laboratory research to field applications. It underscores the importance of moving beyond functional gene characterization to improving the efficiency and scalability of RNAi in real-world agricultural systems. This review systematically lists RNAi target genes and delivery methods in insect pests, identifies research gaps, and supports the development of RNAi-based biopesticides.