Related Experiment Video
Updated: Jun 27, 2026
06:04
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
Published on: February 9, 2016
Modulation of Caffeine Permeation Kinetics in a Skin-PAMPA Model by Probiotic Lysates and Bile Acids
Maja Đanić1, Natalija Dedić2, Dragana Zaklan2
1Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia.
Pharmaceutics
|June 26, 2026
Summary
This study investigated how probiotic lysates and bile acids affect caffeine skin permeation. Probiotic lysates decreased caffeine penetration, while bile acids increased it, offering insights for topical formulation design.
Area of Science:
- Dermatology
- Cosmetic Science
- Pharmacokinetics
Background:
- Caffeine is common in cosmetics but poorly penetrates the skin's stratum corneum.
- Optimizing caffeine delivery is crucial for topical applications.
- This study explores novel delivery enhancement strategies.
Purpose of the Study:
- To investigate the in vitro effects of probiotic bacterial lysates and bile acids on caffeine permeation.
- To analyze the impact on caffeine permeation kinetics.
- To assess potential for tailored topical formulation development.
Main Methods:
- Skin Parallel Artificial Membrane Permeability Assay (Skin-PAMPA) was used for caffeine permeability evaluation.
- High-Performance Liquid Chromatography (HPLC) quantified concentrations over time.
- Apparent permeability coefficients and permeation rates were calculated.
Main Results:
- Probiotic lysates significantly reduced caffeine permeability and altered transport kinetics.
- Bile acids, particularly deoxycholic acid (DCA), increased caffeine apparent permeability.
- Both agents modulated permeation kinetics, not just overall permeability.
Conclusions:
- Probiotic lysates and bile acids differentially modulate caffeine permeation.
- These substances primarily reshape permeation kinetics.
- Findings support the design of topical formulations with controlled caffeine delivery profiles.
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