Quantitative 1H NMR in Pharmaceutical and Biomedical Analyses: Methodologies and Applications
Shangxiao An1, Weiyi Zheng2, Qi Tang2
1School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Molecules (Basel, Switzerland)
|June 26, 2026
Summary
Quantitative 1H NMR (qNMR) offers structural and quantitative analysis without standards. This versatile tool is crucial for pharmaceutical drug analysis and biomedical research, with future advancements promising enhanced capabilities.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Biomedical Science
Background:
- Quantitative 1H NMR (qNMR) is an analytical technique.
- It provides structural and quantitative data without analyte-specific standards.
- qNMR has diverse applications in pharmaceutical and biomedical fields.
Purpose of the Study:
- To review the methodological fundamentals of qNMR.
- To summarize its applications in pharmaceutical and biomedical analysis.
- To discuss emerging technologies and future prospects of qNMR.
Main Methods:
- Review of existing literature on qNMR methodology.
- Analysis of qNMR applications in drug content determination, purity assessment, metabolomics, and cellular process monitoring.
- Discussion of advancements like hyperpolarization, AI, and deep learning.
Main Results:
- qNMR is effective for analyzing small molecules, polysaccharides, polymers, and herbal medicines.
- It is a powerful tool for metabolomics, cellular process monitoring, and metabolite quantification in biofluids and tissues.
- Emerging technologies promise to significantly improve qNMR's sensitivity, resolution, and automation.
Conclusions:
- qNMR is a versatile and powerful analytical tool for both pharmaceutical and biomedical applications.
- Ongoing integration into regulatory frameworks suggests expanding use in quality control and research.
- Future technological advancements will further enhance its capabilities and applications.
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