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Method for Isolating Hypericin from Hypericum perforatum and Preparing Its Micelles for Biomedical Applications.

Dmitry Medvedev1,2, Vasilisa Dalinina3, Polina Golik1

  • 1Department of Chemistry and Technology of Biologically Active Compounds, Medicinal and Organic Chemistry, Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 86 Vernadsky Avenue, 119571 Moscow, Russia.

Molecules (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

Researchers developed a stable, water-soluble hypericin (Hyp) formulation using micelles. This breakthrough enhances Hyp

Keywords:
Pluronic F-127glioblastomahypericinmicellesphotodynamic therapyphotosensitizer

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Area of Science:

  • Natural Products Chemistry
  • Nanotechnology
  • Pharmacology

Background:

  • Hypericin (Hyp) is a natural photosensitizer with therapeutic potential.
  • Hyp's hydrophobicity hinders its medical use.
  • Developing soluble Hyp formulations is crucial for drug delivery.

Purpose of the Study:

  • To efficiently extract hypericin (Hyp) from plant biomass.
  • To create a stable, water-soluble micellar formulation of Hyp.
  • To evaluate the in vitro efficacy of the micellar Hyp formulation.

Main Methods:

  • Soxhlet apparatus for defatting, followed by ultrasonic-assisted extraction with acetone.
  • Thin-film hydration method using Pluronic F-127 to create micelles.
  • In vitro studies using GL261 glioblastoma cells.

Main Results:

  • Micellar formulation achieved high Hyp loading (95.4% EE) and stability (30 nm diameter).
  • Formulation showed efficient cellular uptake and potent photoinduced cytotoxicity against glioblastoma cells.
  • No dark toxicity was observed, indicating safety.

Conclusions:

  • A stable, water-soluble hypericin micellar formulation was successfully developed.
  • The formulation retains Hyp's photosensitizing activity for potential therapies.
  • This method offers a promising approach for photodynamic and sonodynamic therapies.