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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Mitochondria-Targeted Colorimetric and Ratiometric Fluorescent Probe for Hg2+ with Large Stokes Shift.

Dongjian Zhu1, Yufei Zhang1, Yuyan Pan1

  • 1Guangxi Key Laboratory of Health Care Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou 542899, China.

Molecules (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

Researchers developed a novel fluorescent probe for detecting mercury ions (Hg2+). This probe enables sensitive, selective, and visual detection of mercury, even within mitochondria.

Keywords:
Hg2+colorimetric and ratiometricfluorescent probelarge stokes shiftmitochondria-targeted

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Nanotechnology

Background:

  • Mercury ions (Hg2+) pose significant environmental and health risks.
  • Accurate detection of Hg2+ is crucial for environmental monitoring and biological studies.
  • Existing detection methods often lack sensitivity, selectivity, or real-time imaging capabilities.

Purpose of the Study:

  • To develop a novel mitochondria-targeted fluorescent probe for the colorimetric and ratiometric detection of Hg2+.
  • To evaluate the probe's spectral properties, sensitivity, selectivity, and application in biological imaging.

Main Methods:

  • Synthesis of a novel mitochondria-targeted fluorescent probe (Probe 1).
  • Spectroscopic analysis (absorption and fluorescence) upon addition of Hg2+.
  • Evaluation of probe's sensitivity, selectivity, and large Stokes shift.
  • Application of the probe for ratiometric imaging of intracellular Hg2+ in mitochondria.

Main Results:

  • Probe 1 exhibited distinct spectral changes (absorption and fluorescence blue shifts) upon Hg2+ binding.
  • Color change from red to pale yellow (daylight) and red to green (UV lamp) observed.
  • High sensitivity with a limit of detection (LOD) of 25.5 nM and a 824-fold fluorescence intensity ratio variation.
  • Excellent selectivity for Hg2+ over other analytes.
  • Successful ratiometric imaging of intracellular Hg2+ specifically localized within mitochondria.

Conclusions:

  • Probe 1 is a highly sensitive and selective fluorescent probe for Hg2+ detection.
  • The probe's mitochondria-targeting ability and ratiometric response make it suitable for biological imaging.
  • This novel probe offers a valuable tool for monitoring Hg2+ in biological systems.