Related Experiment Video
Updated: Jun 27, 2026
07:05
Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
Published on: August 23, 2024
Brewing-Method-Dependent Changes in the Bioactive Compound Profile and Antioxidant Potential of Coffee Beverages
Magdalena Sęk1, Urszula Cięciel1, Małgorzata Tkacz1
1Department of Food Product Quality, Krakow University of Economics, ul. Sienkiewicza 5, 30-033 Krakow, Poland.
Molecules (Basel, Switzerland)
|June 26, 2026
Summary
Cold brew coffee, rich in chlorogenic acids, offers superior antioxidant potential due to prolonged low-temperature extraction. This method preserves key compounds, enhancing radical-scavenging activity in coffee beverages.
Area of Science:
- Food Chemistry
- Analytical Chemistry
- Nutritional Science
Background:
- Coffee is a significant source of dietary antioxidants.
- The specific compounds driving coffee's antioxidant properties and how brewing impacts them remain unclear.
Purpose of the Study:
- To evaluate the antioxidant potential of individual compounds in coffee beverages.
- To determine the influence of different brewing methods on coffee's bioactive compound profile and antioxidant capacity.
Main Methods:
- Utilized high-performance liquid chromatography coupled with the trolox equivalent antioxidative capacity (TEAC) assay (HPLC-onlineTEAC).
- Analyzed Arabica coffee beverages prepared using 'Americano', 'V60', 'French press', and 'cold brew' methods.
- Assessed caffeine content, chlorogenic acid composition, total phenolic content (TPC), and antioxidant activity.
Main Results:
- Brewing method significantly impacted caffeine, chlorogenic acids, TPC, and antioxidant activity (p < 0.05).
- Cold brew exhibited the highest radical scavenging activity and major caffeoylquinic acid concentrations.
- Chlorogenic acids constituted over 84% of the total antioxidant potential, with monocaffeoylquinic acids being the primary contributors.
Conclusions:
- Prolonged low-temperature extraction (cold brew) enhances coffee's antioxidant potential by preserving reactive chlorogenic acid isomers.
- The brewing method plays a crucial role in the final antioxidant capacity of coffee beverages.
Related Concept Videos
01:25Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
01:22Bioavailability: Influencing Factors
Bioavailability refers to the extent and rate at which a drug reaches systemic circulation in its active form. Extent refers to the amount of the drug that makes it into circulation, while rate is the speed at which it enters circulation. It is influenced by several factors critical for optimizing drug formulations, dosing regimens, and therapeutic outcomes.Physicochemical properties of drugs and formulationsThe solubility, stability, and dissolution rate of a drug significantly impact its...
01:16Experimental Designs
An experimental design is a systematic process that allows researchers to evaluate the relationship between dependent and independent variables. There are three widely used types of experimental design - pre-experimental design, true experimental design, and quasi-experimental design. In pre-experimental design, the researcher compares the data before and after some interventions or treatments. The true-experimental design has more than one purposefully created group, a commonly measured...
01:19Factors Affecting Drug Biotransformation: Biological
Biological factors significantly impact drug metabolism, influencing drug clearance, efficacy, and potential toxicity.
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...
01:23Factors Influencing Bioavailability: First-Pass Elimination
When a drug is taken orally, it undergoes a journey starting from the gastrointestinal (GI) tract, passing through the portal vein, reaching the liver, and finally entering the systemic circulation. This process involves the absorption of the drug across the GI tract. The liver is the primary site for metabolizing the drug, with some metabolism also occurring in the gut wall. This journey significantly reduces the quantity of the drug that reaches the systemic circulation, a phenomenon known as...
01:22Bioavailability Enhancement: Determination and Conceptual Approaches in Overcoming Bioavailability Problems
Bioavailability is a critical pharmacological concept that measures the extent and rate at which an active drug ingredient or therapeutic moiety enters the systemic circulation, remaining unchanged. It's a pivotal factor in determining a drug's efficacy and safety.The Biopharmaceutics Classification System (BCS) plays an essential role in drug development by categorizing drugs into four classes based on their solubility and permeability. This classification aids in understanding drug absorption...