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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: Jun 27, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Reprogramming Rotavirus: Reverse Genetics-Driven Design of Viral Vector Platforms.

Ke Li1, Xiao Wei1,2, Xuanze Ouyang1

  • 1NHC Key Laboratory of Medical Virology and Viral Disease, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Viruses
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

Rotaviruses show promise as oral vaccine vectors due to their safety and ability to induce immunity. Advances in reverse genetics enable the development of new multivalent vaccines using rotaviruses (RV).

Keywords:
oral mucosal vaccinereverse geneticsrotavirusvaccine vector

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Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
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Last Updated: Jun 27, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
15:49

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Published on: June 9, 2022

Area of Science:

  • Virology
  • Vaccinology
  • Molecular Biology

Background:

  • Rotaviruses are a primary cause of severe diarrhea and mortality in young children globally.
  • Oral rotavirus vaccines have proven safe and effective, reducing disease burden and mortality.
  • Rotaviruses possess inherent properties making them suitable as oral mucosal vaccine vectors.

Purpose of the Study:

  • To review the historical development and current applications of rotavirus reverse genetics.
  • To summarize progress in using rotaviruses as a vaccine vector platform for foreign antigens.
  • To critically evaluate challenges and propose a design framework for improved rotavirus-based vectors.

Main Methods:

  • Review of literature on rotavirus reverse genetics and vaccine vector development.
  • Analysis of strategies for foreign gene insertion into the rotavirus genome.
  • Evaluation of immunological outcomes in animal models using rotavirus vectors.

Main Results:

  • Breakthroughs in reverse genetics, including plasmid-based systems, facilitate foreign antigen insertion.
  • Rotavirus vectors have demonstrated potential for delivering foreign antigens and eliciting immune responses.
  • Challenges include optimizing virus rescue efficiency, ensuring genetic insert stability, and addressing animal model limitations.

Conclusions:

  • Rotaviruses represent a promising platform for developing novel oral mucosal vaccines.
  • Further research is needed to overcome current challenges in rotavirus vector development for enhanced stability and immunogenicity.
  • Rational design of rotavirus-based vectors is crucial for future vaccine applications.