Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inhibitors of Viral Protein Synthesis

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

Inhibitors of Virion Maturation and Assembly

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Are Suicide Prevention Interventions Effective for Current and Ex-Serving Military Personnel? A Systematic Review and Meta-Analysis Based on the Suicide Prevention Trials Database.

Suicide & life-threatening behavior·2026

Same author

Multimorbidity and suicidality among military veterans: a scoping review.

Psychiatry research·2026

Same author

The lived experience of clinical instructors as simulated patients: a qualitative study.

Journal of clinical education in physical therapy·2025

Same author

Impact of theatre interventions on mental health: a systematic literature review and meta-analysis.

Journal of epidemiology and community health·2025

Same author

ZPR1 Is Dispensable for HPV R-Loop Resolution but Regulates Host R-Loop Dynamics.

Viruses·2025

Same author

The Association Between Body Composition and Chemotherapy-Induced Toxicity in Pancreatic Cancer: A Systematic Review.

ANZ journal of surgery·2025

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Targeted Protein Degradation Strategies in DNA Virus Research.

Michael Lam¹, Chayah Hill¹, Ethan Thornburg¹

¹Tom and Julie Wood College of Osteopathic Medicine, Marian University, Indianapolis, IN 46222, USA.

|June 26, 2026

Summary

Proteolysis Targeting Chimeras (PROTACs) offer a novel approach to combat DNA viruses by selectively degrading viral or host proteins. This technology enables precise control over host protein expression and direct targeting of viral proteins for therapeutic benefit.

Keywords:

DNA viruses PROTACs antiviral viral lifecycles

More Related Videos

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Area of Science:

Molecular Biology
Virology
Drug Discovery

Background:

DNA viruses utilize host cellular machinery for replication, complicating the study and targeting of viral proteins.
Existing methods like CRISPR and siRNA have limitations in targeting host proteins involved in viral lifecycles.

Purpose of the Study:

To explore Proteolysis Targeting Chimeras (PROTACs) as a novel strategy for targeting DNA viral proteins and host factors.
To investigate the application of PROTACs for temporal control of host protein expression in DNA viral research.
To highlight PROTACs as potential antivirals against DNA viruses and virus-associated malignancies.

Main Methods:

PROTACs are heterobifunctional molecules that recruit E3 ubiquitin ligases to induce target protein degradation.
Utilizing PROTACs to modulate host proteins for studying DNA viral processes.
Employing PROTACs as direct antivirals targeting viral proteins.

Main Results:

PROTACs enable selective and rapid degradation of target proteins, including viral and host factors.
This approach allows for temporal control over host protein expression, aiding in the investigation of viral mechanisms.
PROTACs show promise in targeting previously undruggable proteins and developing new antiviral therapies.

Conclusions:

PROTACs represent a powerful and versatile tool for both investigating DNA viral lifecycles and developing novel antiviral therapeutics.
The technology offers a promising avenue for targeting virus-associated malignancies and overcoming challenges in antiviral drug development.
Further research into host-targeting and viral-targeting PROTACs can significantly advance DNA virus research and treatment strategies.