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Porcine Erythrocyte-PRRSV Interactions: Implications for Targeted Nanodrug Delivery

Wei Yin1, Jingze Li1, Haoxiang Yao1

  • 1Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.

Veterinary Sciences
|June 26, 2026
PubMed

Insights

Researchers developed a targeted nanodrug delivery system for porcine reproductive and respiratory syndrome virus (PRRSV). This system utilizes porcine erythrocytes and CR1-like interactions to deliver matrine nanoliposomes, showing promise for PRRSV control.

Area of Science:

  • Veterinary Virology
  • Nanomedicine
  • Immunology

Background:

  • Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat to the swine industry.
  • Understanding PRRSV infection mechanisms is crucial for developing effective control strategies.
  • Erythrocytes play a role in immune responses and pathogen interactions.

Purpose of the Study:

  • To establish a CR1-like-mediated targeted nanodrug delivery system for PRRSV.
  • To investigate the interactions between porcine erythrocytes, PRRSV, and nanodrugs.
  • To evaluate the efficacy of matrine nanoliposomes for PRRSV control.

Main Methods:

  • Determined optimal conditions for PRRSV sensitization with porcine serum.
  • Verified CR1-like-dependent immune adhesion of erythrocytes to PRRSV using microscopy and molecular techniques.
  • Assessed the impact of this adhesion on PRRSV infection of porcine alveolar macrophages (PAMs).
  • Prepared and characterized mannose-modified matrine nanoliposomes (MMLNPs) for in vitro evaluation.

Main Results:

  • PRRSV sensitization was optimized under specific conditions (37°C, 2h).
  • Porcine erythrocytes demonstrated specific CR1-like-mediated adhesion to sensitized PRRSV, enhancing PAM infection.
  • Developed MMLNPs were stable, non-cytotoxic, and targeted PAMs via CR1-like.
  • MMLNPs exhibited superior in vitro antiviral activity compared to free matrine.

Conclusions:

  • CR1-like-mediated immune adhesion is a key mechanism in PRRSV infection of PAMs.
  • Targeting this natural pathway allows for efficient delivery of nanodrugs to infected cells.
  • This approach offers a promising strategy for developing novel PRRSV therapeutics.
Abstract

Keywords:
PAMsPRRSVmannosematrineporcine CR1-like

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