Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Depletion and replacement of tissue resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus.

Development (Cambridge, England)·2026

Same author

Bovine natural killer cell subsets can be defined by differential expression of CD161 (<i>KLRB1</i>).

Frontiers in immunology·2026

Same author

Homozygous kinase-dead Csf1r mutation in outbred mice reveals essential and redundant functions of tissue resident macrophages.

Journal of leukocyte biology·2026

Same author

Artificial light at night disrupts expression of circadian and metabolic genes in the green anole lizard (Anolis carolinensis).

Proceedings. Biological sciences·2026

Same author

Corneal retention using a nanorobot-based eyedrop.

Journal of materials chemistry. B·2025

Same author

Interleukin 10 controls the balance between tolerance, pathogen elimination, and immunopathology in birds.

eLife·2025

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

CSF1R-dependent macrophages control B cell development and function in the chicken immune system.

Zhiguang Wu¹, Rakhi Harne¹, Alewo Idoko-Akoh¹

¹The Roslin Institute, The University of Edinburgh, Edinburgh, United Kingdom.

Journal of Immunology (Baltimore, Md. : 1950)

|June 26, 2026

Summary

This summary is machine-generated.

In chickens, a specific macrophage population expressing CSF1R and SPIC differentiates to function as follicular dendritic cells, crucial for adaptive immunity. Loss of CSF1R impairs macrophage development, leading to immune deficiencies in birds.

Keywords:

B cell CSF1R follicular dendritic cells macrophage

More Related Videos

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Published on: March 18, 2019

Related Experiment Videos

Last Updated: Jun 27, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Published on: March 18, 2019

Area of Science:

Immunology
Cell Biology
Avian Biology

Background:

Mammalian acquired immunity relies on antigen-presenting cells like macrophages and follicular dendritic cells.
Follicular dendritic cells in mammals are critical for germinal center function and B cell maturation.
Chicken cells expressing CSF1R (colony-stimulating factor 1 receptor) were investigated for macrophage and dendritic cell roles.

Purpose of the Study:

To identify and characterize CSF1R-expressing cell populations in chicken spleen.
To determine the function of CSF1R+ macrophages in avian adaptive immunity.
To investigate the role of CSF1R in macrophage development and immune organ function in chickens.

Main Methods:

Single-cell RNA sequencing of chicken spleen CSF1R+ cells.
Generation of CSF1R knockout chickens using germline editing.
Analysis of immune cell populations, lymphoid organ development, and B cell deficiency in mutant birds.

Main Results:

Two distinct macrophage populations were identified: TIMD4/C1Q/MAFB+ (involved in antigen capture) and MARCO/VSIG4+/SPIC+ (located in follicles).
CSF1R knockout chickens lacked embryonic macrophages, failed to thrive post-hatch, and exhibited monocytopenia and granulocytosis.
Loss of CSF1R led to follicular macrophage depletion in the bursa, resulting in organ involution and severe B cell deficiency.

Conclusions:

A specialized CSF1R+, SPIC+ macrophage population in birds differentiates to function as follicular dendritic cells.
CSF1R is essential for avian macrophage development, immune cell homeostasis, and the maintenance of lymphoid organ structure and function.
Disruption of CSF1R impacts adaptive immunity by causing B cell deficiency due to follicular macrophage loss.