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Related Experiment Video

Updated: Jun 27, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

Published on: August 4, 2018

Alternative Polyadenylation Dynamics During the Rice Blast Immune Response.

Dewei Yang1,2, Xinyi Li3, Haohua He3

  • 1Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.

Molecular Plant Pathology
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

Alternative polyadenylation (APA) shortens gene transcripts during rice blast infection, potentially evading miRNA repression. This regulation offers new strategies for controlling rice diseases.

Keywords:
PAT‐seqalternative polyadenylationmiRNA targetsmiRNA‐seqpost‐transcriptional regulationrice blast

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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Last Updated: Jun 27, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

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Published on: August 4, 2018

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Published on: June 2, 2021

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Plant pathology
  • Molecular biology
  • Genomics

Background:

  • Alternative polyadenylation (APA) is a key post-transcriptional regulatory mechanism controlling 3' untranslated region (UTR) lengths.
  • Understanding APA dynamics during plant-pathogen interactions is crucial for disease resistance.

Purpose of the Study:

  • To investigate genome-wide APA profile changes in rice during Magnaporthe oryzae infection.
  • To explore the relationship between APA, gene expression, and microRNA (miRNA) regulation in rice blast.
  • To functionally characterize APA dynamics in rice-pathogen interactions.

Main Methods:

  • Poly(A)-tag sequencing (PAT-seq) was employed to profile APA dynamics.
  • Gene expression and miRNA expression levels were analyzed.
  • CRISPR/Cas9 was used for functional characterization of a candidate gene.

Main Results:

  • Widespread APA-driven transcript shortening was observed during M. oryzae infection, particularly in stress-response genes.
  • 3' UTR shortening correlated negatively with gene expression and may facilitate immune gene evasion of miRNA repression.
  • Functional analysis of Os05g0509500 revealed its positive role in rice blast susceptibility.

Conclusions:

  • APA is a tightly regulated process during rice blast, influencing gene expression and miRNA interactions.
  • Transcriptome-wide APA site selection and its interplay with miRNAs offer novel insights into rice disease resistance.
  • Understanding APA mechanisms provides a basis for developing new strategies to control rice blast.