Targeting Oral Tissue Regeneration With Standardized Centella asiatica Extract: Experimental Insights From Human...

Area of Science:

• Biotechnology
• Cell Biology
• Dermatology

Background:

• Centella asiatica is a medicinal plant with potential therapeutic properties.
• Assessing the safety of plant extracts is crucial before clinical application.
• Human dermal fibroblasts are key cells in wound healing and tissue regeneration.

Purpose of the Study:

• To evaluate the in vitro cytotoxic effects of Centella asiatica extract on human dermal fibroblasts.
• To determine the basic safety and biocompatibility of Centella asiatica extract for oral tissue regeneration.
• To establish dose-response relationships for Centella asiatica extract's effect on cell viability.

Main Methods:

• Human dermal fibroblasts were cultured in Dulbecco's Modified Eagle Medium.
• Cell viability was assessed using the MTT assay at various extract concentrations (5-200 µg/mL).
• Statistical analysis included one-way ANOVA and nonlinear regression, with Triton X-100 as a positive control.

Main Results:

• Centella asiatica extract did not significantly reduce human fibroblast viability at any tested concentration.
• No concentration-dependent cytotoxic effect was observed, even up to 200 µg/mL.
• Cell viability remained stable and non-cytotoxic, unlike the positive control (Triton X-100).

Conclusions:

• Centella asiatica extract is biocompatible and non-cytotoxic to human dermal fibroblasts up to 200 µg/mL.
• The extract demonstrates an excellent safety profile, supporting its use in further studies.
• Findings support the potential application of Centella asiatica in oral soft tissue regeneration and wound healing.