Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-binding landscape of amiloride: large-scale profiling and structural basis of U-U mismatch recognition.

RSC chemical biology·2026
Same author

Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes.

Nature communications·2026
Same author

"More" Artificial mRNAs: Beyond the Art of Nature.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

RNA-binding fluorogenic probes: G-clamp conjugated with a thiazole orange derivative for screening RNA-binding small molecules.

RSC chemical biology·2025
Same author

Nematode telomerase RNA hitchhikes on introns of germline-up-regulated genes.

Science (New York, N.Y.)·2025
Same author

Visualization of liquid-liquid phase transitions using a tiny G-quadruplex binding protein.

Nature communications·2025

Related Experiment Video

Updated: Jun 28, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

RNA-Protein complexes and their role in cell fate.

Kaito Masaki1, Malvin Leonardo Pardi1, Hirohide Saito1,2,3

  • 1Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

Physiology (Bethesda, Md.)
|June 26, 2026
PubMed
Summary

RNA-protein interactions regulate gene expression through post-transcriptional control, impacting cell fate and disease. This review explores RNA-protein complexes, from defined assemblies to biomolecular condensates, and their role in health and pathology.

Keywords:
Cell fate transitionRNA-protein complexRNP complex

More Related Videos

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Related Experiment Videos

Last Updated: Jun 28, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • RNA-protein interactions are crucial for post-transcriptional gene regulation.
  • These interactions influence fundamental cellular processes, including stem cell self-renewal, differentiation, stress response, and senescence.
  • Dysregulation of RNA-protein interactions is linked to various pathological conditions.

Purpose of the Study:

  • To review the spectrum of RNA-protein complexes, bridging defined stoichiometric assemblies and non-stoichiometric biomolecular condensates.
  • To discuss the connection between these complexes via RNA-protein interactions and RNA modification.
  • To highlight the pathological implications of aberrant RNA-protein complex formation and explore future research directions and therapeutic opportunities.

Main Methods:

  • Literature review and synthesis of current research on RNA-protein complexes.
  • Discussion of the continuum from defined complexes (e.g., ribosomes, spliceosomes, miRISCs) to biomolecular condensates (e.g., stress granules, processing bodies).
  • Exploration of the role of RNA modification in modulating these interactions.

Main Results:

  • RNA-protein complexes exist on a biological continuum, not as strictly separate entities.
  • RNA-protein interactions and modifications are key connectors across this spectrum.
  • Abnormalities in these complexes contribute to disease development.

Conclusions:

  • Understanding the RNA-protein complex continuum is vital for comprehending gene regulation.
  • Further research into RNA-protein interactions and modifications may reveal novel therapeutic targets.
  • Investigating novel experimental approaches is essential for advancing the field and developing new treatments.