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Updated: Jun 28, 2026
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Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Cellular Dynamics and Dentine Bridge Formation Following Direct Pulp Capping With MTA and Biodentine in a Murine
Amnah Sajdeya1, Joshua Milgram2, Nurit Kot-Limon3
1The Institute of Biomedical and Oral Research, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
International Endodontic Journal
|June 26, 2026
Summary
Biodentine significantly enhanced dentine bridge formation and pulp healing compared to Mineral Trioxide Aggregate (MTA) in a direct pulp capping (DPC) murine model. Biodentine demonstrated superior regenerative potential, suggesting it as a more effective dental regenerative material.
Area of Science:
- Biomaterials Science
- Regenerative Dentistry
- Dental Pulp Biology
Background:
- Direct pulp capping (DPC) aims to preserve pulp vitality through mineralized dentine bridge formation.
- Mineral Trioxide Aggregate (MTA) and Biodentine are leading DPC materials, but comparative in vivo regenerative data is limited.
Purpose of the Study:
- To compare the dentine bridge formation and pulp response of MTA versus Biodentine in a controlled murine DPC model.
- To elucidate the underlying mechanisms of pulp regeneration influenced by these biomaterials.
Main Methods:
- DPC was performed on murine molars using MTA or Biodentine.
- Evaluated dentine bridge formation via micro-CT, histology, and fluorochrome incorporation.
- Assessed pulp response using immunohistochemistry, RNAscope, TUNEL assay, and EdU labeling.
Main Results:
- Biodentine induced significantly greater dentine bridge volume and mineral content than MTA.
- Histology revealed more complete bridges with fewer defects in Biodentine-treated teeth.
- Biodentine promoted reduced apoptosis, enhanced cell retention, and increased odontoblast activity compared to MTA.
Conclusions:
- Biodentine significantly outperformed MTA in promoting dentine bridge formation and pulp regeneration in a murine DPC model.
- Findings suggest Biodentine's superiority is linked to reduced apoptosis, better cell retention, and stimulated odontoblast activity.
- This study highlights the utility of murine models for investigating pulp healing mechanisms and supports Biodentine as a potent dental regenerative agent.
