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Published on: February 23, 2024
Temperature Range Thermal Proteome Profiling for Drug Target Identification: A Practical Guide
Yi Xue1,2, Peiyu Chen2, Meining Xing2
1State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai district, Tianjin301617, China.
Journal of Proteome Research
|June 26, 2026
Summary
Thermal proteome profiling (TPP) identifies drug targets. This review details thermal range TPP (TPP-TR) evolution, methods, and data analysis, offering strategies for improved drug discovery and mechanism studies.
Area of Science:
- Pharmacology
- Biochemistry
- Proteomics
Background:
- Identifying drug targets and off-targets is essential for understanding drug mechanisms.
- Thermal proteome profiling (TPP) is a direct, simple, and scalable method for target identification.
- TPP can be applied at the live-cell level to reveal pathway regulation.
Purpose of the Study:
- To systematically review the evolution of thermal range TPP (TPP-TR).
- To discuss the advantages and limitations of TPP-TR sample preparation and data analysis.
- To propose strategies for developing and applying innovative TPP-TR methods.
Main Methods:
- Review of existing literature on TPP, focusing on TPP-TR.
- Analysis of TPP-TR sample preparation techniques.
- Evaluation of TPP-TR data analysis methodologies.
Main Results:
- TPP-TR has evolved significantly, becoming a widely practiced TPP format.
- Current TPP-TR methods have specific advantages and limitations in sample prep and data analysis.
- Improvements in TPP-TR can enhance its application in drug discovery.
Conclusions:
- TPP-TR is a valuable tool for drug target identification and mechanism elucidation.
- Addressing current limitations in TPP-TR sample preparation and data analysis is crucial.
- Further innovation in TPP-TR methods will advance drug discovery research.
