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Updated: Jun 28, 2026
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
Eu3+-mediated ratiometric fluorescence visualization sensing for traceability and content prediction of plant-derived
Xueqing Zeng1, Guanghua Lei2, Yun Huang2
1Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central Minzu University, Wuhan, China; The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China.
Abstract:
Plant-derived products are rich in a variety of flavonol derivative (Flav), of which the types and contents are closely related to its origin. This work amplified the Flav content differences between different origins of plant-derived products by MPA@CuNPs@Eu3+-CTC nano-sensing system. In the presence of Flav, fluorescence intensity of MPA@CuNPs is restored. Meanwhile, Flav hinders the "antenna effect" process between Eu3+ and CTC, reducing the fluorescence intensity of Eu3+ and thus forming a ratio fluorescence sensor. Ultimately, in the presence of Flav of different concentrations, rich color changes are presented. Furthermore, by analyzing the RGB data through Random Forest, the classification accuracy rate of Chrysanthemi Flos (CF) from different origins achieved 100 %. Moreover, the regression coefficients of content prediction results ranged from 0.87 to 0.98 (RMSE < 0.13). This strategy provides a new approach for on-site immediate origin traceability and quality monitoring of high-value plant-based products rich in flavonol.
