Generation of a FRMD5 knockout human embryonic stem cell line by CRISPR/Cas9 editing

FRMD5 is a protein-coding gene on human chromosome 15q15.3 encoding a FERM domain-containing protein. Its mutations are linked to NEDEMA, a rare autosomal dominant neurodevelopmental disorder characterized by developmental delay, intellectual disability, ataxia, epilepsy, and eye movement abnormalities (Keller Sarmiento et al., 2024).We established a FRMD5 knockout (FRMD5^-/^-) human embryonic stem cell line via CRISPR/Cas9. This line shows normal karyotype, expresses pluripotency markers, and differentiates into three germ layers, serving as a valuable tool for studying FRMD5 in neural development.