Related Experiment Video

Updated: Jun 28, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

HnRNPK promotes SVCV replication by stabilizing viral phosphoprotein and targeting IRF3 to suppress type I interferon

Yanwei Zhang1, Xin Lin1, Mingyi Li1

  • 1College of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, 464000, China.

Fish & Shellfish Immunology
|June 26, 2026
PubMed

Insights

Heterogeneous nuclear ribonucleoprotein K (hnRNPK) promotes viral replication in fish by stabilizing viral proteins and suppressing the host interferon response. This protein offers a potential target for antiviral strategies in aquatic animals.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a key RNA-binding protein involved in cellular processes.
  • Its specific role in fish antiviral immunity and viral replication is not well understood.

Purpose of the Study:

  • To investigate the function of hnRNPK in common carp antiviral immunity against SVCV.
  • To elucidate the molecular mechanisms by which hnRNPK influences viral replication and host immune responses.

Main Methods:

  • Analyzing hnRNPK expression levels in SVCV-infected carp.
  • Investigating hnRNPK's interaction with viral proteins and host immune factors.
  • Assessing the impact of hnRNPK overexpression on viral replication and interferon production.

Main Results:

  • hnRNPK expression increased upon SVCV infection and translocated to the cytoplasm.
  • hnRNPK overexpression enhanced viral replication by stabilizing the SVCV phosphoprotein (P).
  • hnRNPK suppressed type I interferon production by targeting IRF3, inhibiting its nuclear translocation and promoting degradation.

Conclusions:

  • hnRNPK promotes SVCV replication via dual mechanisms: stabilizing the viral P protein and suppressing the type I interferon response through IRF3.
  • hnRNPK plays a significant role in SVCV immune evasion strategies.
  • hnRNPK represents a potential therapeutic target for antiviral interventions in fish.
Keywords:
HnRNPKIRF3InterferonSVCV

More Related Videos

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...