Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational design and synthesis of Co(ii), Ni(ii) and Cu(ii) complexes bearing 1,2,4-triazole scaffold for biological applications.

RSC advances·2026
Same author

Directed C-H activation of 13α-estrone: a pathway to promising AKR1C inhibitors <i>via</i> docking and biological studies.

RSC advances·2026
Same author

Light-Driven Competitive Selection in a Protein-Catalyzed Dissipative Peptide Replication.

Angewandte Chemie (International ed. in English)·2026
Same author

Copper(II), a Peculiar Metal Ion for Complexation With Monensin A Ionophore.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Characterization and Biofungicide Potential of a Novel Antifungal defensin, K4CBP6, from Solanum lycopersicum L.

Probiotics and antimicrobial proteins·2025
Same author

Beyond plasma membrane disruption: Novel antifungal mechanism of Neosartorya (Aspergillus) fischeri antifungal protein 2 in Candida albicans.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Jun 28, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Mimicking LPMO Active Sites with Synthetic copper(II) Complexes: Exploring the Minimal Requirements.

Azza A Hassoon1, Nóra V May2, Zoltán Kele3

  • 1Chemistry Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.

Inorganic Chemistry
|June 26, 2026
PubMed
Summary

Copper(II) complexes with histamine and Schiff-base ligands mimic lytic polysaccharide monooxygenases (LPMOs). Hydroxyl radicals generated by these complexes efficiently catalyze substrate conversion, with simpler histamine complexes showing higher activity.

More Related Videos

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

Related Experiment Videos

Last Updated: Jun 28, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

Area of Science:

  • Bioinorganic Chemistry
  • Biomimetic Catalysis
  • Enzyme Mechanisms

Background:

  • Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes crucial for biomass degradation.
  • Developing functional models of LPMOs is essential for understanding their catalytic mechanisms and for biotechnological applications.
  • Copper(II) complexes with histamine (Hm) and Schiff-base ligands (L) were investigated as LPMO mimics.

Purpose of the Study:

  • To synthesize and characterize copper(II) complexes of histamine and a Schiff-base ligand.
  • To evaluate their activity as functional models of LPMOs.
  • To elucidate the catalytic mechanism, particularly the role of hydroxyl radicals.

Main Methods:

  • X-ray crystallography
  • pH-potentiometry
  • UV-vis spectroscopy
  • Electron Paramagnetic Resonance (EPR) spectroscopy
  • Enzyme kinetics with p-nitrophenyl-β-d-glucopyranoside (PNPG)

Main Results:

  • Structural characterization of Cu(II)-L species confirmed stability across a wide pH range (2-10).
  • Copper(II) complexes generated hydroxyl radicals in the presence of hydrogen peroxide (HO).
  • Both Cu(II)-Hm and Cu(II)-L systems efficiently modeled LPMO activity at pH 7.4 and 10.5, with Cu(II)-Hm complexes exhibiting higher catalytic efficiency.
  • Hydroxyl radical was identified as the key reactive species in the catalytic cycle.

Conclusions:

  • Simpler copper(II)-histamine complexes can serve as effective LPMO mimics.
  • Tridentate T-shaped coordination is not essential for efficient LPMO-like molecular catalysts.
  • These findings suggest new avenues for designing robust and simple LPMO-mimicking catalysts for practical applications.