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How deep is deep enough? The importance of the detection limits of the CML minimal residual disease gDNA assay

Giovanni Micheloni1,2, Vittoria Moretti1, Annalisa Frattini3,2

  • 1Dipartimento di Medicina e Chirurgia, Università dell'Insubria, 21100, Varese, Italy.

Cell Death Discovery
|June 26, 2026
PubMed
Abstract

No abstract available in PubMed .

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Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...