Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-destructive transcriptomics via vesicular export.

Nature communications·2026
Same author

Gim3 buffers and potentiates de novo mutations that affect fluconazole susceptibility in yeast.

EMBO reports·2026
Same author

High Cell Density Fermentation of <i>Yarrowia lipolytica</i> on n-Hexadecane for the Valorization of Pyrolyzed Plastic Waste.

International journal of molecular sciences·2026
Same author

Author Correction: The Solve-RD Solvathons as a pan-European interdisciplinary collaboration to diagnose patients with rare disease.

Nature genetics·2026
Same author

scooby: modeling multimodal genomic profiles from DNA sequence at single-cell resolution.

Nature methods·2025
Same author

Nucleotide dependency analysis of genomic language models detects functional elements.

Nature genetics·2025

Related Experiment Video

Updated: Jun 28, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
12:02

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 18, 2014

Intron location and sequence modulate gene expression in Yarrowia lipolytica.

Qi Qi1,2, Pedro Tomaz da Silva3,4, Vasileios Vangalis1,2

  • 1Centre of Microbial and Plant Genetics (CMPG), Department of Microbial and Molecular Systems (M2S), KU Leuven, Leuven 3000, Belgium.

Nucleic Acids Research
|June 27, 2026
PubMed
Summary
This summary is machine-generated.

Researchers identified key intron features that regulate gene expression in Yarrowia lipolytica. These findings enable precise control of gene expression for microbial cell factories.

More Related Videos

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Related Experiment Videos

Last Updated: Jun 28, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
12:02

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 18, 2014

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Introns are prevalent in eukaryotic genomes and often correlate with higher gene expression.
  • The specific intron features that influence gene expression are not well understood.
  • Yarrowia lipolytica is a valuable oleaginous yeast for industrial applications.

Purpose of the Study:

  • To systematically characterize the intron landscape of Yarrowia lipolytica.
  • To identify intron features that impact splicing efficiency and gene expression.
  • To explore the potential of introns as regulatory elements in microbial cell factories.

Main Methods:

  • Transcriptome analysis across 12 diverse environments.
  • Identification and annotation of introns and intron-containing genes.
  • Linear regression modeling to correlate intron features with gene expression levels.
  • Reporter gene assays to test the regulatory effects of selected introns.

Main Results:

  • 2421 introns identified in 1430 genes, including 1302 novel introns.
  • Intron-containing genes showed higher and more stable expression across conditions.
  • Six key intron features (splice motifs, branch point, GC content, size) were found to influence expression.
  • A predictive model demonstrated introns can modulate reporter gene expression up to 200-fold.
  • A novel intron (I3) was identified that significantly enhances gene expression and protein production.

Conclusions:

  • Intron features significantly influence splicing efficiency and gene expression.
  • Introns can be effectively utilized as tunable regulatory elements in Yarrowia lipolytica.
  • These findings offer new strategies for optimizing gene expression in microbial cell factories.