Related Experiment Video
Updated: Jun 28, 2026
08:55
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
Published on: March 17, 2018
Rice-Derived Peptides Exhibit Wound Healing Properties in Human Keratinocytes: Screening, Identification, and
Tingmin Qu1, Shiyu Wen1, Liangjie Hu1
1School of Food Science and Bioengineering, Hunan Provincial Key Laboratory of Cytochemistry, Changsha University of Science & Technology, Changsha, 410114, P.R. China.
Plant Foods for Human Nutrition (Dordrecht, Netherlands)
|June 27, 2026
Summary
Rice protein peptides show promise for skin repair. A specific peptide, PG9, enhances keratinocyte migration and reduces inflammation by interacting with epidermal growth factor receptor (EGFR) and regulating key signaling pathways.
Area of Science:
- Biochemistry
- Dermatology
- Molecular Biology
Background:
- Food-derived biopeptides offer health benefits and safety.
- Rice protein hydrolysates are explored for therapeutic potential.
- Keratinocyte injury models are crucial for studying skin repair mechanisms.
Purpose of the Study:
- Investigate rice protein hydrolysates and derived peptides for protective effects against inflammation in human keratinocytes.
- Identify specific peptides with skin repair capabilities.
- Elucidate the molecular mechanisms underlying peptide-mediated skin repair.
Main Methods:
- Rice protein hydrolysis and peptide isolation.
- Cell viability and migration assays on HaCaT cells.
- LC-MS/MS and in-silico analysis for peptide identification.
- Molecular dynamics simulations and pathway analysis (PI3K/AKT/mTOR).
Main Results:
- Rice protein hydrolysates enhanced keratinocyte viability and migration.
- Five peptides were identified, with PG9 exhibiting significant activity.
- PG9 downregulated pro-inflammatory cytokines (RANTES, IL-1β, IL-6, IL-23) and promoted keratinocyte migration.
- PG9 stabilized within the epidermal growth factor receptor (EGFR) binding site and modulated the PI3K/AKT/mTOR pathway.
Conclusions:
- PG9 is a potent EGFR-binding peptide derived from rice protein.
- PG9 demonstrates anti-inflammatory effects and promotes skin repair signaling.
- PG9 holds potential as a natural agent for wound healing and skin regeneration.
Related Concept Videos
01:19Clinical Applications of Epidermal Stem Cells
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:11Healing I: Introduction
Healing is the physiological process by which the body restores the integrity and function of damaged tissues following injury. It involves a coordinated interplay of cellular proliferation, extracellular matrix remodeling, and growth factor signaling. The extent and nature of the tissue damage determine whether healing occurs by resolution, regeneration, or replacement.ResolutionResolution represents the most complete form of healing, occurring when the injury is minimal and tissue...
01:28Phases of Wound Repair
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...