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Updated: Jun 29, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Two new meroterpenoids with 6/10/5/3 tetracyclic skeleton from fungus Ampulloclitocybe clavipes
Zhaocui Sun1, Qiao Wu1, Mingzhen Liu1
1National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Abstract:
Two new meroterpenoids, clavilactones R (1) and S (2) with 6/10/5/3 tetracyclic skeleton, and one known meroterpenoid clavilactone E were isolated from the fungus Ampulloclitocybe clavipes. Their chemical structures, including absolute configurations, were unambiguously determined through HR-ESI-MS, 1D/2D-NMR spectral data and ECD calculations. All three compounds were evaluated for their in vitro cytotoxic activities. Among them, clavilactone S (2) exhibited moderate cytotoxic activity against HGC-27 and A549 tumour cell lines, with IC50 values of 19.2 and 24.8 µM, respectively. Clavilactone R (1) showed weak cytotoxicity, while clavilactone E (3) displayed no obvious cytotoxic effect (IC50 > 50 μM) against both cell lines.
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