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Robinin Attenuates Xenobiotic Enzymes, Inflammation, and Apoptosis on DMH-Induced Colon Cancer via Modulating
Wang Anlei1, Wang Kaihao1, Gong Yazhao1
1Department of General Surgery, Xingtai People's Hospital, Xingtai, Hebei Province, China.
Journal of Biochemical and Molecular Toxicology
|June 28, 2026
Summary
Robinin (RB) effectively reduced colorectal cancer (CRC) development in a rat model. This natural compound decreased tumor growth and pre-cancerous lesions by regulating inflammation and apoptosis.
Area of Science:
- Oncology
- Pharmacology
- Natural Products Chemistry
Background:
- Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide.
- Developing novel therapeutic strategies for CRC is essential due to treatment challenges.
- Understanding CRC's molecular underpinnings is key to identifying new therapeutic targets.
Purpose of the Study:
- To investigate the anticancer effects of Robinin (RB) on 1,2-dimethylhydrazine (DMH)-induced CRC in a rat model.
- To evaluate RB's impact on tumor development, pre-cancerous lesions, and related molecular pathways.
- To assess RB's potential as a therapeutic agent for colorectal cancer.
Main Methods:
- Rats were induced with CRC using 1,2-dimethylhydrazine (DMH) and treated with varying doses of Robinin (RB) for 15 weeks.
- Tumor volume, number, aberrant crypt foci (ACF), crypt multiplicity, and body weight were assessed.
- Levels of inflammatory cytokines, histopathological alterations, and protein expression (Bax, Caspase-3, NF-κB) were analyzed.
- Hepatic enzyme activities and cAMP levels were measured to evaluate metabolic and signaling pathway modulation.
Main Results:
- DMH-induced rats showed a 100% incidence of tumors and pre-cancerous lesions.
- RB treatment significantly reduced tumor volume, number, ACF, and crypt multiplicity in a dose-dependent manner.
- RB diminished weight loss, inflammatory markers, and histopathological damage, while restoring cAMP levels and enzyme activities.
Conclusions:
- Robinin (RB) demonstrates significant anticancer properties against DMH-induced colorectal cancer in rats.
- RB exerts its effects by modulating inflammation, apoptosis, and key signaling pathways (NF-κB, PI3K/Akt/mTOR/Ras).
- Robinin shows promise as a potential therapeutic agent for colorectal cancer prevention and treatment.
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