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Published on: August 23, 2019
Interconnected oxidative and nitrosative reactions in fermented sausages during storage: Modulation by red barberry
Hülya Serpil Kavuşan1, Meltem Serdaroğlu1
1Ege University, Engineering Faculty, Food Engineering Department, 35100 Bornova, Izmir, Turkey.
None:
This study investigated the effects of red barberry extract (RBE) on lipid oxidation, protein oxidation, and nitrosative reactions in fermented sausages during refrigerated storage. RBE was obtained using ultrasound-assisted extraction optimized by response surface methodology and characterized by high phenolic content and antioxidant activity. Fermented sausages were produced with four formulations: control (C) and treatments containing 200, 300, and 400 ppmRBE (S-BR2, S-BR3, S-BR4). Vacuum-packaged samples were stored at 4 °C for 4 months and analyzed monthly for peroxide value, TBARS, protein carbonyls, sulfhydryl groups, residual nitrite, 3-nitrotyrosine, volatile N-nitrosamines, color parameters, and sensory properties. Storage significantly increased lipid oxidation and protein oxidation markers, while sulfhydryl groups and residual nitrite decreased during storage (P < 0.05). RBE addition significantly reduced peroxide value, TBARS, and protein carbonyl formation and preserved sulfhydryl groups compared with the control. The highest extract level (400 ppm) showed the strongest protective effect and resulted in lower accumulation of volatile N-nitrosamines at the end of the storage. Sensory evaluation indicated improved oxidative stability without negative effects on overall acceptability. Principal component analysis and hierarchical clustering revealed clear grouping patterns among oxidative and nitrosative indicators and demonstrated that storage time was the main factor influencing overall variability, while RBE moderated the intensity of these changes. These findings indicate that RBE can effectively improve oxidative stability and reduce nitrosamine formation in fermented sausages during storage.
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