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Method for needle-free release of pathogens from host cells using reusable chromatography components.
Frank Seeber1, Sharareh Salehi Hossainy1, Christoph-Martin Ufermann2
1FG 16 - Mycotic and Parasitic Agents and Mycobacteria, Robert Koch-Institute, Berlin, Germany.
Journal of Microbiological Methods
|June 28, 2026
Summary
Researchers developed a new, economical device for releasing intracellular pathogens from host cells. This needle-free method minimizes infectious aerosols and risks associated with traditional cell lysis techniques.
Area of Science:
- Biotechnology
- Cell Biology
- Microbiology
Background:
- Intracellular pathogen release often uses "syringing" through needles.
- This method poses risks of needle sticks and infectious aerosol generation.
Purpose of the Study:
- To develop a novel, safe, and economical method for host cell lysis.
- To minimize risks associated with intracellular pathogen release.
Main Methods:
- Assembly of a reusable host cell lysis device from chromatography components.
- Utilized a needle-free approach for manual cell disruption.
Main Results:
- The device provides a needle-free method for cell lysis.
- The method significantly minimizes the generation of infectious aerosols.
- The device is economical and reusable.
Conclusions:
- A new, cost-effective, and safe device for host cell lysis has been developed.
- This innovation reduces occupational hazards and aerosol risks in pathogen research.
