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A Barcoding Strategy for Pooled Single-Cell LA-ICP-TOFMS Analysis of Metal-Containing Therapeutics.

Claude Molitor1,2,3, Lyndsey Hendriks1, Antonia Hafner4,5

  • 1Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna 1090, Austria.

Analytical Chemistry
|June 29, 2026
PubMed
Summary
This summary is machine-generated.

A new barcoding strategy enables simultaneous analysis of multiple experiments using laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS). This method efficiently assesses metal-based therapeutics like oxaliplatin and BOLD-100 in cancer cells.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Cell Biology

Background:

  • Laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) offers cellular resolution for metal-based therapeutics.
  • Current methods for screening metal-containing anticancer agents are time-consuming and labor-intensive.

Purpose of the Study:

  • To develop a barcoding strategy for simultaneous analysis of multiple experiments using LA-ICP-TOFMS.
  • To assess the uptake of BOLD-100 and oxaliplatin in colorectal cancer cells, including oxaliplatin-resistant lines.

Main Methods:

  • A barcoding approach using wheat germ agglutinin (WGA) and lanthanide-labeled anti-WGA antibodies was employed.
  • Pooled samples were analyzed via LA-ICP-TOFMS and processed using the MeXpose single-cell data pipeline.
  • The strategy was applied to compare drug uptake in HCT116 wild-type (WT) and oxaliplatin-resistant (OxR) cells.

Main Results:

  • Pooling 10 experiments reduced consumable use, measurement time, and processing time while improving data consistency.
  • Barcoding-enabled analysis revealed significantly lower oxaliplatin uptake in OxR cells compared to WT cells.
  • BOLD-100 uptake was minimally affected by oxaliplatin resistance.

Conclusions:

  • The developed barcoding strategy enables efficient and scalable assessment of metal-based therapeutics.
  • This method facilitates high-throughput screening and comparative analysis of drug uptake in different cellular contexts.