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Updated: Jun 30, 2026
09:11
Capsular Serotyping of Streptococcus pneumoniae by Latex Agglutination
Published on: September 25, 2014
Development of a Serotyping Scheme for Streptococcus pasteurianus: An Underreported Zoonotic Pathogen
Shuoyue Wang1,2,3, Xinchun Li1,2,3, Chenxu Zheng1,2,3
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210014, China, njau.edu.cn.
Transboundary and Emerging Diseases
|June 29, 2026
Summary
A new serotyping scheme for Streptococcus pasteurianus was developed using multiplex PCR (mPCR). This method accurately identifies five serotypes, aiding in tracking this zoonotic pathogen in humans and animals.
Area of Science:
- Microbiology
- Veterinary Medicine
- Infectious Diseases
Background:
- Streptococcus pasteurianus is an opportunistic zoonotic pathogen causing severe infections in humans and animals.
- It is a significant cause of swine streptococcosis.
- A lack of a systematic serotyping scheme hinders epidemiological studies and control efforts.
Purpose of the Study:
- To establish a systematic serotyping scheme for Streptococcus pasteurianus.
- To develop and validate a multiplex PCR (mPCR) assay for rapid serotyping.
- To support improved surveillance and control of S. pasteurianus infections.
Main Methods:
- Six representative S. pasteurianus strains were used for antiserum production.
- Agglutination assays identified five distinct serotypes (1a, 1b, 2, 3, and 4).
- A multiplex PCR (mPCR) assay was developed based on cps gene cluster variations and validated with 35 isolates.
Main Results:
- Five serotypes of S. pasteurianus were identified using agglutination assays.
- The developed mPCR assay demonstrated full concordance with serological typing.
- Transmission electron microscopy confirmed the presence of a capsular layer in all serotypes.
Conclusions:
- A robust serotyping framework for S. pasteurianus has been established.
- The mPCR serotyping assay is a valuable tool for epidemiological studies and diagnostics.
- This framework will enhance surveillance and control of S. pasteurianus infections.
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