Related Experiment Video
Updated: Jun 30, 2026
09:39
Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
Published on: February 7, 2021
Polysaccharide-Structured Nanoemulgel Matrix Regulates the Co-delivery, Retention, and Biointerface Activity of
Geethika Manohar1, Priyanka Srivastava1
1Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore, Tamil Nadu 632 014, India.
ACS Omega
|June 29, 2026
Summary
This study developed a dual-drug nanoemulgel for enhanced skin regeneration. The formulation effectively eliminated wound biofilms and promoted skin cell healing, showcasing its therapeutic potential.
Area of Science:
- Biomaterials Science
- Dermatology
- Nanotechnology
Background:
- Skin regeneration and antimicrobial treatments are crucial for wound healing.
- L-ascorbic acid (AA) and L-lysine (L) are key compounds for skin health and repair.
- Developing stable and effective drug delivery systems for these compounds is essential.
Purpose of the Study:
- To develop a stable dual-drug water-in-oil (W/O) nanoemulsion encapsulating L-ascorbic acid (AA) and L-lysine (L).
- To formulate a tragacanth-based nanoemulgel for enhanced skin regeneration and antimicrobial efficacy.
- To evaluate the nanoemulgel's stability, drug encapsulation, biofilm disruption, and effects on keratinocytes and wound closure.
Main Methods:
- Optimization of W/O nanoemulsion for AA and L.
- Incorporation into a tragacanth-based nanoemulgel.
- Characterization of droplet size, polydispersity index, and zeta potential.
- Stability testing under accelerated and real-time conditions.
- Encapsulation efficiency determination.
- Antimicrobial activity assessment (bacterial viability and biofilm disruption) using crystal violet assay and FE-SEM.
- Cytotoxicity evaluation on HaCaT keratinocytes.
- In vitro wound closure assay.
Main Results:
- Optimized AA/L W/O nanoemulsion achieved a droplet size of ~150 nm with good stability.
- High encapsulation efficiencies for AA (94.63%) and L (91%) in nanoemulsion, further increased in nanoemulgel (97.75% for AA, 95.13% for L).
- The nanoemulgel demonstrated significant reduction in bacterial viability and disrupted mono- and dual-species biofilms.
- Formulation was non-cytotoxic to HaCaT keratinocytes and significantly promoted in vitro wound closure.
Conclusions:
- Codelivery of AA and L in a biopolymer-stabilized W/O nanoemulgel is a promising strategy for wound treatment.
- The nanoemulgel effectively eliminates wound biofilms and promotes skin regeneration.
- This formulation offers enhanced antimicrobial and skin healing properties for advanced wound care applications.
