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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
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SLC2A9-Mediated Uric Acid Homeostasis Modulates Apoptosis in TNBC

Peng Chen1, Jiapeng Xu1, Yulong Liang1

  • 1Department of Thyroid, Breast and Hernia Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Breast Cancer (Dove Medical Press)
|June 29, 2026
PubMed
Abstract

Insights

The solute carrier family 2, member 9 (SLC2A9) gene is downregulated in triple-negative breast cancer (TNBC). SLC2A9 regulates uric acid (UA) metabolism, inhibiting TNBC cell growth and metastasis.

Area of Science:

  • Oncology
  • Metabolic pathways
  • Molecular biology

Background:

  • Triple-negative breast cancer (TNBC) exhibits aggressive behavior and limited therapeutic options.
  • Uric acid (UA) metabolism is increasingly recognized for its role in cancer progression.
  • The specific role of SLC2A9 in TNBC and its connection to UA metabolism require further investigation.

Purpose of the Study:

  • To elucidate the biological function of SLC2A9 in TNBC.
  • To determine the molecular mechanisms by which SLC2A9 influences TNBC cell behavior through UA metabolism.

Main Methods:

  • Bioinformatics analysis identified intersecting genes in TNBC and UA metabolism.
  • In vitro studies established SLC2A9 overexpression and knockdown models in TNBC cell lines.
  • Cellular assays assessed proliferation, apoptosis, migration, and invasion; Western blot analyzed protein expression.

Main Results:

  • SLC2A9 expression was significantly reduced in TNBC tissues and cell lines.
  • SLC2A9 overexpression suppressed TNBC cell proliferation, migration, and invasion, while enhancing apoptosis.
  • SLC2A9 modulated intracellular and extracellular UA levels, impacting apoptosis-related and UA metabolism proteins.

Conclusions:

  • SLC2A9 plays a critical role in regulating the malignant phenotype of TNBC by altering UA homeostasis.
  • SLC2A9's anticancer effects are linked to its UA transport function.
  • SLC2A9 presents potential as a prognostic biomarker and a novel metabolic therapeutic target for TNBC.
Keywords:
SLC2A9TNBCapoptosisurate transportersuric acid metabolism

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