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A programmable benchtop photocrosslinking chamber for controlled bioconjugation.

Samuel Olson1, Gulsu Sener1, Alan Weisgerber1

  • 1Cancer Early Detection Advanced Research Center (CEDAR), Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA.

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Summary

Researchers developed a low-cost, open-source UV irradiation system for bioconjugation. This user-friendly device efficiently photo-crosslinks biomolecules in Eppendorf tubes with reduced sample heating.

Keywords:
BioconjugationPhotochemistryPhotocrosslinkingPhotoreactorUltraviolet

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Area of Science:

  • Biomedical Sciences
  • Photochemistry
  • Molecular Biology

Background:

  • Ultraviolet (UV) photochemical reactions, particularly photo-crosslinking, are vital in biomedical research for applications like drug discovery and bioprinting.
  • Existing UV irradiation sources are often bulky, expensive, or cause excessive sample heating, limiting their practical use in experiments.
  • Current UV systems lack user-friendliness for specific applications like bioconjugation in Eppendorf tubes.

Purpose of the Study:

  • To present an open-source, low-cost, and customizable UV irradiation system for bioconjugation.
  • To address the limitations of existing UV sources in terms of cost, size, user-friendliness, and sample heating.
  • To enable efficient and precise photochemical reactions in standard laboratory consumables.

Main Methods:

  • Development of a modular UV irradiation system utilizing 365 nm UV LEDs arranged for uniform light distribution.
  • Incorporation of a polished aluminum sleeve to enhance light distribution within 1.5 mL Eppendorf tubes.
  • Testing the system's efficiency and impact on sample temperature compared to commercial mercury lamp systems.

Main Results:

  • The developed system is low-cost (approx. $250) and processes up to six Eppendorf tubes simultaneously.
  • Achieved higher efficiency and a 50% reduction in sample heating compared to commercial systems.
  • Demonstrated efficient conjugation of photocrosslinkable nanobodies to antibodies in 5 minutes without photobleaching.

Conclusions:

  • The open-source UV system offers a user-friendly, cost-effective, and efficient alternative for bioconjugation and other photochemical reactions.
  • The modular design allows for customization with different wavelengths for broader photoreaction applications.
  • The system facilitates advanced biomedical applications such as immunofluorescent imaging by enabling rapid, low-heating bioconjugation.