Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Astragalus membranaceus</i> and <i>Salvia miltiorrhiza</i> Inhibit Tryptase/PAR2-Mediated Mast Cells - Peritoneal Mesothelial Cells Crosstalk to Ameliorate Peritoneal Fibrosis.

Journal of inflammation research·2026
Same author

Antioxidant Activity of Chlorogenic Acid Evaluated via EPR Spectroscopy and Its Visual Tracking in Mouse Kidney.

Nutrients·2026
Same author

Insights into Antioxidant Activity and Trace Element Distribution of Aqueous Extract of <i>Silybum marianum</i> Seeds.

Molecules (Basel, Switzerland)·2026
Same author

Unveiling the Therapeutic Potential of Suyin Detoxification Prescription in Diabetic Nephropathy: A Comprehensive Study Integrating Clinical Efficacy, Network Pharmacology, and Molecular Mechanisms.

Journal of diabetes research·2026
Same author

Didymin halts knee osteoarthritis progression by targeting the GSK3B/5-LOX/11-HETE pathway to suppress chondrocyte ferroptosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

A Method for Preparing Morphologically Preserved Wildlife Fecal Specimens for Long-Term Ecological Studies.

Ecology and evolution·2026

Related Experiment Video

Updated: Jun 30, 2026

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Diosgenin Promotes Bacterial Clearance in Bladder Epithelial Cells by Regulating Rab27b Expression: A Study Based on

Hao Yin1,2, Jiamin Yin2, Jiahao Zhang2

  • 1No. 1 Clinical Medical College, Nanjing University of Chinese Medicine (Jiangsu Province Hospital of Chinese Medicine), Nanjing, 210000, People's Republic of China.

Infection and Drug Resistance
|June 29, 2026
PubMed
Summary

Diosgenin (DSG) targets Rab27b to treat urinary tract infections (UTIs). This natural compound enhances bacterial clearance by upregulating Rab27b protein, offering a non-antibiotic therapeutic approach for UPEC infections.

Keywords:
diosgeninintracellular bacteriaurinary tract infectionuropathogenic Escherichia colivirtual screening

More Related Videos

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
08:53

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Published on: December 4, 2020

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
09:50

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Published on: October 18, 2019

Related Experiment Videos

Last Updated: Jun 30, 2026

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
08:53

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Published on: December 4, 2020

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
09:50

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Published on: October 18, 2019

Area of Science:

  • Microbiology
  • Pharmacology
  • Biochemistry

Background:

  • Antimicrobial resistance (AMR) necessitates novel non-antibiotic therapies for infectious diseases.
  • Urinary tract infections (UTIs), often caused by uropathogenic Escherichia coli (UPEC), involve intracellular bacterial survival within bladder epithelial cells (BECs).
  • Rab27b facilitates the expulsion of intracellular bacteria, with its upregulation shown to reduce bacterial loads.

Purpose of the Study:

  • To identify compounds that bind to Rab27b using virtual screening.
  • To investigate the therapeutic potential of diosgenin (DSG) in treating UPEC-induced UTIs by modulating Rab27b.
  • To elucidate the mechanism by which DSG affects intracellular bacterial clearance and bladder tissue integrity.

Main Methods:

  • Virtual screening and molecular dynamics simulations for compound identification.
  • Surface plasmon resonance (SPR) to confirm direct binding of DSG to Rab27b.
  • Cellular assays (CCK-8, Western blot, immunofluorescence, colony counting, siRNA) and in vivo mouse models (HE staining, SEM) to assess DSG's effects.

Main Results:

  • Diosgenin (DSG) and ST5330591 (ST) were identified as stable binders of Rab27b.
  • DSG upregulated Rab27b protein expression post-transcriptionally, enhancing intracellular UPEC clearance in BECs.
  • DSG treatment in a mouse UTI model reduced intracellular bacterial CFU, alleviated inflammation, and protected bladder epithelial integrity.

Conclusions:

  • DSG directly binds to Rab27b and upregulates its protein expression via a post-transcriptional mechanism.
  • This upregulation promotes the clearance of intracellular UPEC from BECs, demonstrating therapeutic potential for UTIs.
  • DSG represents a promising non-antibiotic therapeutic agent for managing UPEC-induced UTIs.