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Updated: Jun 30, 2026

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
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Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

A UCP1-IRES-Cre Knock-In Mouse Enables Specific Brown Adipocyte Targeting Without CNS Off-Target Expression.

Joshua Meyer, Jaclyn Williams, Jennifer Bailey

    Research Square
    |June 29, 2026
    PubMed
    Summary
    This summary is machine-generated.

    A new UCP1-IRES-Cre mouse model accurately targets thermogenic adipocytes, reducing off-target Cre activity seen in older models. This improved specificity aids research into energy metabolism and obesity.

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    Last Updated: Jun 30, 2026

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    Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

    Published on: June 12, 2020

    Area of Science:

    • Metabolic research
    • Adipocyte biology
    • Genetics

    Background:

    • Uncoupling protein 1 (UCP1) is crucial for thermogenesis in brown adipocytes.
    • Existing BAC-transgenic UCP1-Cre mouse lines show unwanted Cre activity in non-adipose tissues.
    • This limits their reliability for studying thermogenic adipocytes.

    Purpose of the Study:

    • To characterize a novel knock-in UCP1-IRES-Cre mouse model.
    • To assess its specificity in targeting thermogenic adipocytes compared to BAC-transgenic models.
    • To evaluate Cre activity across various tissues and its correlation with UCP1 protein expression.

    Main Methods:

    • Generated UCP1-IRES-Cre knock-in mice.
    • Crossed UCP1-IRES-Cre mice with Ai14 reporter mice for tdTomato visualization.
    • Compared reporter expression patterns with endogenous UCP1 protein localization via immunohistochemistry.
    • Analyzed Cre activity in adipose tissue, brain, and peripheral organs.

    Main Results:

    • Reporter expression in UCP1-IRES-Cre mice closely matched endogenous UCP1 distribution in brown and white adipose tissue.
    • Significantly reduced ectopic Cre activity was observed compared to BAC-transgenic models.
    • Minimal reporter expression occurred in the hypothalamus and peripheral organs, except for the choroid plexus.
    • White adipose depots showed broader reporter labeling than UCP1 staining, suggesting developmental promoter activity.

    Conclusions:

    • The UCP1-IRES-Cre knock-in model accurately reflects endogenous UCP1 expression patterns.
    • This model offers improved specificity for genetic targeting of thermogenic adipocytes.
    • It presents a valuable alternative to BAC-transgenic models for metabolic research.