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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Laparoscopic Ovum Pick-up For In Vivo Oocyte Retrieval In Alpacas.

Julio Landinez-Aponte1, Myeongdon Joo1, Rusty Stott1

  • 1Department of Animal, Dairy and Veterinary Sciences, S.J. and Jessie E. Quinney College of Agriculture and Natural Resources, Utah State University.

Journal of Visualized Experiments : Jove
|June 29, 2026
PubMed
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Laparoscopic ovum pick-up (LOPU) offers a viable method for retrieving alpaca oocytes. This minimally invasive technique achieved a 61.3% oocyte recovery rate, supporting reproductive biotechnology applications.

Area of Science:

  • Reproductive Biology
  • Veterinary Medicine
  • Biotechnology

Background:

  • Alpacas possess unique reproductive and immunological traits, making them valuable models for biomedical research.
  • Their induced ovulation and distinct immune system, featuring heavy-chain-only antibodies, facilitate comparative and translational studies.
  • Laparoscopic ovum pick-up (LOPU) is a minimally invasive technique for oocyte recovery in species with complex reproductive anatomy.

Purpose of the Study:

  • To develop and standardize a reproducible in vivo oocyte retrieval protocol in alpacas utilizing LOPU.
  • To establish LOPU as a viable method for oocyte recovery in alpaca reproductive biotechnology.

Main Methods:

  • Eight adult female alpacas underwent monthly Laparoscopic ovum pick-up (LOPU) sessions.

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Retrieval of Mouse Oocytes
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Retrieval of Mouse Oocytes

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Last Updated: Jul 1, 2026

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Published on: May 11, 2017

Retrieval of Mouse Oocytes
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Published on: April 28, 2007

  • Ovarian stimulation involved gonadotropin-releasing hormone and follicle-stimulating hormone administration.
  • Oocytes were retrieved laparoscopically under general anesthesia using a three-port approach and aspirated follicles via a vacuum system.
  • Main Results:

    • A mean of 14.88 ± 3.85 follicles were observed per alpaca.
    • An average of 10.94 ± 3.62 follicles were aspirated per session.
    • The study achieved a mean oocyte recovery rate of 61.30 ± 8.21%.

    Conclusions:

    • Laparoscopic ovum pick-up (LOPU) is a feasible and reproducible technique for in vivo oocyte retrieval in alpacas.
    • This method holds potential for advancing reproductive biotechnology workflows in camelids.
    • The established protocol supports further research utilizing alpaca oocytes.