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Related Experiment Video

Updated: Jul 1, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatially Resolved, Integrated Single-Cell Multiomic Profiling of the Transcriptome and Epigenomic Targets in Frozen

Ting Zhang1, Fatemeh S Farassati1, Alejandro Stark-Quiroz1

  • 1Enteric Neuroscience Program and Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine and Science; Gastroenterology Research Unit, Division of Gastroenterology and Hepatology, Department of Medicine, Mayo Clinic College of Medicine and Science.

Journal of Visualized Experiments : Jove
|June 29, 2026
PubMed
Summary

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This study introduces a spatial multiomic method combining epigenomic and transcriptomic profiling in single brain cells. This approach enhances cell identification and reveals spatial regulatory programs within tissues.

Area of Science:

  • Molecular Biology
  • Genomics
  • Neuroscience

Background:

  • Spatial multiomic technologies and single-cell profiling are crucial for understanding complex tissue architecture.
  • Current methods require efficient workflows for spatially resolved molecular analysis of cells.

Purpose of the Study:

  • To develop and present a spatial cleavage under targets & tagmentation (CUT&Tag) multiome workflow.
  • To simultaneously profile histone H3K27ac modification and gene expression within a single frozen brain section.

Main Methods:

  • Spatial barcoding of nuclei in cryosections embedded in optimal cutting temperature (OCT) medium.
  • Single-nucleus isolation followed by CUT&Tag assay.
  • Combined capture of tagmented DNA, RNA, and spatial barcodes for library preparation and sequencing.

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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

Related Experiment Videos

Last Updated: Jul 1, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

Main Results:

  • Generation of spatially annotated epigenomic and transcriptomic profiles from the same nuclei.
  • Assignment of multiomic information to specific anatomical coordinates within the tissue.
  • Improved cell-type identification fidelity by integrating epigenomic and spatial transcriptomic data.

Conclusions:

  • The developed workflow enables simultaneous spatial profiling of epigenomic and transcriptomic data.
  • Integration of multiomic data enhances the understanding of cell-cell interactions and regulatory programs in situ.
  • This method facilitates detailed molecular and cellular analysis of complex tissues with spatial resolution.