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Related Experiment Video

Updated: Jul 1, 2026

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
07:44

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

Published on: November 28, 2019

Plate-Based Imaging Assay for Quantifying Photoperiodic Responses in Neurospora crassa Using Protoperithecia

Aleece Siner1, Kwangwon Lee2

  • 1Center for Computational and Integrative Biology (CCIB) Rutgers, The State University of New Jersey.

Journal of Visualized Experiments : Jove
|June 29, 2026
PubMed
Summary
This summary is machine-generated.

The Protoperithecia Assay (PPA) quantifies fungal development under light-dark cycles. This method offers a reproducible workflow for studying photoperiodic responses in model organisms like Neurospora crassa.

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Last Updated: Jul 1, 2026

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Area of Science:

  • Mycology
  • Developmental Biology
  • Chronobiology

Background:

  • Neurospora crassa is a key model organism for biological timing.
  • Understanding light-regulated development is crucial in fungal biology.
  • Existing methods for assessing photoperiodic responses lack standardization.

Purpose of the Study:

  • To establish a reproducible and scalable workflow for assessing fungal sexual development under controlled photoperiods.
  • To quantify photoperiodic responses in Neurospora crassa using protoperithecia formation.
  • To provide a standardized method for comparative analysis of fungal development.

Main Methods:

  • Fungal cultures grown on synthetic crossing medium under controlled light-dark cycles.
  • Standardized removal of surface conidia to enhance visualization of protoperithecia.
  • Manual image-based counting of protoperithecia for quantitative analysis.

Main Results:

  • The Protoperithecia Assay (PPA) integrates standardized culture conditions and controlled photoperiod exposure.
  • Quadrant-based imaging enables reproducible quantitative comparisons across fungal strains.
  • The workflow provides a consistent and scalable framework for measuring photoperiod-dependent development.

Conclusions:

  • The PPA offers a simple and accessible platform for comparative analysis of photoperiod-dependent development in fungi.
  • This method requires approximately one week from inoculation to analysis.
  • The PPA facilitates reproducible research on fungal responses to light-dark cycles in laboratory settings.