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Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: Jul 1, 2026

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels

Dot Blot Assay for Detecting Global N⁶-Methyladenosine RNA Modification Levels

Published on: February 6, 2026

Decoding The Epitranscriptome: In Silico Insights Into m6A Regulatory Network In Breast Cancer.

Sarah Abdulkarim¹, Salwa Akhtar¹, Mati Ur Rehman²

¹Department of Life Sciences, College of Science and General Studies, Alfaisal University.

Journal of Visualized Experiments : Jove

|June 29, 2026

Summary

This summary is machine-generated.

N6-methyladenosine (m6A) regulators are crucial in breast cancer. This study provides a bioinformatics framework to analyze m6A regulators, identifying key genes linked to patient survival, paving the way for new therapies.

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Last Updated: Jul 1, 2026

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels

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Published on: February 6, 2026

MeRIP-qPCR Assay for Detecting m6A Modification Levels of Specific RNA in Osteosarcoma Cells

MeRIP-qPCR Assay for Detecting m⁶A Modification Levels of Specific RNA in Osteosarcoma Cells

Published on: December 30, 2025

Area of Science:

Epitranscriptomics
Cancer Genomics
Bioinformatics

Background:

N6-methyladenosine (m6A) is the most abundant internal RNA modification, vital for RNA metabolism and gene expression.
Dysregulation of m6A regulators is implicated in cancer, but their specific roles in breast cancer require comprehensive understanding.

Purpose of the Study:

To provide a step-by-step bioinformatics framework for analyzing m6A regulators in breast cancer using public datasets.
To assess the mutational landscape, gene expression, and prognostic relevance of m6A regulators in breast cancer.

Main Methods:

Utilized publicly available datasets from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) project.
Performed mutational analyses and gene expression profiling of m6A regulators.
Systematically evaluated the association between m6A regulatory components and patient survival.

Main Results:

Identified distinct patterns of genetic alterations and differential expression among key m6A regulators in breast cancer.
Found that METTL14, CBLL1, YTHDC1, HNRNPC, HNRNPA2B1, and RBMX are associated with better patient survival.
Determined that YWHAG is associated with poor overall survival in breast cancer patients.

Conclusions:

This study offers a systems genomics overview of m6A regulatory genes in breast cancer.
Demonstrated a practical and reproducible bioinformatics workflow for analyzing epitranscriptomic regulators.
Findings support the development of novel m6A-based diagnostic and therapeutic strategies for breast cancer.