Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut-derived IL-17A via STAT3/RORγt signaling underlies sleep disruption-induced depression: Targeting effects of Schisandrin B therapy.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Screening of a novel VHH pair targeting different epitopes of D-dimer and development of a sandwich luminex immunoassay for plasma D-dimer detection.

Analytica chimica acta·2026
Same author

Ultrafast Polarization Modulation Driven by above-Bandgap Photoexcitation in Ferroelectric NbOCl<sub>2</sub>.

The journal of physical chemistry letters·2026
Same author

Screening for novel variable domains of the heavy chains of heavy-chain antibodies targeting different epitopes of thrombomodulin and Luminex fluorescence immunoassay for soluble thrombomodulin.

International journal of biological macromolecules·2025
Same author

Acrylamide Neurotoxicity Studies in <i>Caenorhabditis elegans</i> Model.

Antioxidants (Basel, Switzerland)·2025
Same author

Traditional Chinese Medicine Improves the Development of Vascular Thrombotic Inflammation: The Key Role of Gut Microbiota and Its Derived Metabolites.

Phytotherapy research : PTR·2025

Related Experiment Video

Updated: Jul 1, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

A Chemiluminescence Assay for Differentiating MicroRNA Isoforms with 3'-Terminal Single-Nucleotide Resolution.

Yuelan He1, Huizhu Song1, Yingtung Lo1

  • 1School of Pharmaceutical Sciences, Fudan University, Laboratory of Smart Drug Delivery, MOE, State Key Laboratory of Advanced Drug Formulations for Overcoming Delivery Barriers, Shanghai 201203, China.

Analytical Chemistry
|June 29, 2026
PubMed
Summary

This study introduces a novel chemiluminescent method for detecting 3'-terminal isomiRs, which are microRNA variants. The technique offers high sensitivity and specificity for identifying single-nucleotide differences, aiding disease biomarker discovery.

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Jul 1, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • MicroRNAs (miRNAs) are crucial noncoding RNAs regulating gene expression.
  • IsomiRs, terminal length variants of miRNAs, arise from imprecise processing and can impact biological pathways.
  • Single-nucleotide variations in isomiRs present challenges for accurate detection but hold potential as disease biomarkers.

Purpose of the Study:

  • To develop a highly sensitive and specific method for discriminating 3"-terminal single-nucleotide isomiRs.
  • To overcome limitations of conventional techniques in identifying subtle isomiR variations.
  • To enable precise detection of isomiRs for potential biomarker applications.

Main Methods:

  • A chemiluminescent strategy utilizing T4 RNA ligase 2 and melting temperature-based discrimination.
  • Design of a stem-loop hairpin probe for selective ligation of perfectly matched miRNAs.
  • Detection of ligation products via a biotinylated reporter probe and streptavidin-horseradish peroxidase (SA-HRP) for chemiluminescence.

Main Results:

  • The method achieved precise, single-nucleotide resolution in discriminating 3"-terminal isomiRs.
  • Demonstrated superior sensitivity and specificity compared to conventional techniques.
  • Quantifiable chemiluminescent signal generated from specific ligation events.

Conclusions:

  • The developed chemiluminescent strategy provides a robust tool for accurate isomiR detection.
  • This approach enhances the potential of isomiRs as reliable biomarkers for various diseases.
  • The method addresses the challenge of high-fidelity discrimination of single-nucleotide isomiRs.