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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
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UCA1 lncRNA regulates γ-globin expression by modulating the miR-148b/BCL11A axis.

Motiur Rahaman1, Shatarupa Bhattacharya1, Mandrita Mukherjee1

  • 1School of Medical Science and Technology, IIT Kharagpur, Kharagpur, India.

Life Science Alliance
|June 29, 2026
PubMed
Summary
This summary is machine-generated.

A novel regulatory axis involving long noncoding RNA UCA1 and microRNA 148b is crucial for hemoglobin switching. This mechanism sustains BCL11A expression, ensuring proper adult hemoglobin production and preventing fetal hemoglobin persistence.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Hematology

Background:

  • Hemoglobin switching from fetal to adult forms is vital for oxygen transport.
  • Dysregulation of this process contributes to beta-hemoglobinopathies.
  • BCL11A is a known repressor of fetal gamma-globin, but posttranscriptional regulation is unclear.

Purpose of the Study:

  • To elucidate the posttranscriptional mechanisms regulating BCL11A expression during erythropoiesis.
  • To investigate the role of long noncoding RNA UCA1 and microRNA 148b in globin gene switching.

Main Methods:

  • Identified miR-148b as a direct regulator of BCL11A.
  • Demonstrated UCA1 acts as a molecular decoy for miR-148b.
  • Assessed the impact of UCA1 depletion and ectopic expression on BCL11A and gamma-globin levels.

Main Results:

  • UCA1 sequesters miR-148b, preventing repression of BCL11A.
  • UCA1 depletion leads to increased miR-148b, decreased BCL11A, and induced gamma-globin.
  • Ectopic UCA1 restores BCL11A and silences gamma-globin.

Conclusions:

  • A novel UCA1/miR-148b regulatory axis fine-tunes hemoglobin switching.
  • This axis stabilizes BCL11A, reinforcing gamma-globin silencing in adult erythroid cells.
  • Uncovered a lncRNA-mediated mechanism integrating miRNA and transcriptional control in erythropoiesis.