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Published on: July 7, 2023
A Nanopore Direct RNA Sequencing Atlas of RNA Modifications in pig (Sus scrofa domesticus) Testes across Sexual
Yiting Yang1,2,3, Zhijuan Yan1,2,3, Ziling Hao1,2,3
1Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Abstract:
RNA modifications represent a crucial layer of post-transcriptional regulation, yet their dynamic landscape during testicular maturation remains largely uncharted. In this study, we employed Nanopore Direct RNA Sequencing to generate a comprehensive epitranscriptomic dataset for porcine testicular tissue at pre-sexual and post-sexual maturation stages. This dataset captures the profiles of four RNA modifications (m⁶A, m⁵C, Ψ, and inosine) across these critical developmental time points. We further characterized the global abundance, distribution, and motif features of these modifications. This resource provides a valuable foundation for elucidating the epitranscriptomic mechanisms governing mammalian testicular development and spermatogenesis.
