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The Soft Agar Colony Formation Assay
Published on: October 27, 2014
CCT7 Expression Affects the Prognosis of Lung Adenocarcinoma
Yue Li1, Longqian Wei1, Guiyu Feng1
1Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Background:
CCT7 is a chaperonin containing tailless complex polypeptide 1 subunit. The study focused on investigating its prognostic significance for lung adenocarcinoma (LUAD).
Methods:
CCT7 expression in LUAD and matched non-carcinoma lung samples was examined via TCGA database. Besides, we utilized the GEO database and RT-qPCR for validating differential CCT7 expression. The relation with clinical factors and its functions in diagnosis and prognosis prediction were determined. GO, KEGG, along with GSEA were conducted for exploring CCT7's functions. We also performed CIBERSORT, ssGSEA, and ESTIMATE for examining the LUAD immune microenvironment. By analyzing tumor mutational burden (TMB) and immune checkpoints, the relationship between CCT7 and immunotherapy was explored.
Results:
CCT7 expression significantly increased within LUAD relative to matched non-carcinoma lung samples. As revealed by GO, KEGG, as well as GSEA results, CCT7 was mostly related to cell movement and regulation of multiple biological processes. Furthermore, CCT7 expression varied significantly among different immune cells, and those having increased CCT7 expression showed greater TMB.
Conclusions:
An increase in CCT7 expression carries significant implications for diagnosing and predicting LUAD prognosis.
