Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Rab Cascades

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative Metabolome and Transcriptome Analysis Revealed the Accumulative Mechanism of Rubusoside in Chinese Sweet Tea.

Journal of agricultural and food chemistry·2024

Same author

Corrigendum to "CsMIEL1 effectively inhibits the accumulation of anthocyanins under low temperatures in tea plants (Camellia sinensis)" [Plant Physiol. Biochem. 221 (2024) PLAPHY_108726].

Plant physiology and biochemistry : PPB·2024

Same author

CsMIEL1 effectively inhibits the accumulation of anthocyanins under low temperatures in tea plants (Camelliasinensis).

Plant physiology and biochemistry : PPB·2024

Same author

A flavonoid metabolon: cytochrome b<sub>5</sub> enhances B-ring trihydroxylated flavan-3-ols synthesis in tea plants.

The Plant journal : for cell and molecular biology·2024

Same author

Competition between FLS and DFR regulates the distribution of flavonols and proanthocyanidins in <i>Rubus chingii</i> Hu.

Frontiers in plant science·2023

Same author

Functional analysis of the dihydroflavonol 4-reductase family of <i>Camellia sinensis</i>: exploiting key amino acids to reconstruct reduction activity.

Horticulture research·2022

Same journal

Genome-wide identification and expression analysis of the Glutathione S-transferase (GST) gene family in Avicennia marina under salt and auxin stress.

BMC plant biology·2026

Same journal

Novel HD-Zip transcription factor PCD8 integrates developmental gene networks to shape rice panicle morphogenesis.

BMC plant biology·2026

Same journal

An analysis of pruning practices in soilless tomato cultivation.

BMC plant biology·2026

Same journal

Whole-genome analysis reveals the tandem duplication of Cannabis sativa L. GATA and their stress-responsive expression during seed germination.

BMC plant biology·2026

Same journal

Exogenous gibberellin promotes lateral branch development in stumping Pinus yunnanensis by regulating endogenous hormones and TCP genes.

BMC plant biology·2026

Same journal

Predicting capsicum leaf water stress using mid-infrared ATR-FTIR spectroscopy.

BMC plant biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2026

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Published on: March 11, 2020

The RcMYB21 transcription factor activates flavonol biosynthesis by targeting RcFLS in Chinese raspberry.

Ting Lei^1,2, Jun Huang^1,3, Wei Qian¹

¹School of Life Sciences, Anhui Agricultural University, Hefei, China.

BMC Plant Biology

|June 29, 2026

Summary

This summary is machine-generated.

We identified the RcMYB21 transcription factor in Chinese raspberry, which regulates flavonoid production and enhances salt stress tolerance. This discovery sheds light on the plant

Keywords:

Chinese raspberry Flavonoid biosynthesis Flavonol MYB transcription factor Secondary metabolism

More Related Videos

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Related Experiment Videos

Last Updated: Jul 1, 2026

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Published on: March 11, 2020

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Area of Science:

Plant Molecular Biology
Biochemistry
Genetics

Background:

Chinese raspberry (Rubus chingii Hu) is a valuable medicinal plant in traditional Chinese medicine.
Flavonoids are key bioactive compounds in raspberries, but their biosynthesis regulation is poorly understood.

Purpose of the Study:

To identify and characterize transcription factors involved in regulating flavonoid biosynthesis in Rubus chingii.
To investigate the role of RcMYB21 in flavonoid production and its potential function in stress response.

Main Methods:

Identification and cloning of the RcMYB21 gene.
Gene expression analysis using RT-qPCR.
Subcellular localization, transactivation assays, Y1H, and dual-luciferase assays.
Overexpression studies in raspberry, tobacco, and Arabidopsis.

Main Results:

RcMYB21, an R2R3-MYB transcription factor, was identified and confirmed to have nuclear localization and transactivation activity.
RcMYB21 directly binds to and activates the promoter of RcFLS1, a key gene in flavonol biosynthesis.
Overexpression of RcMYB21 led to increased flavonol accumulation and altered anthocyanin levels, demonstrating its role in flavonoid regulation.
RcMYB21-overexpressing Arabidopsis showed enhanced lateral root development and improved salt stress tolerance.

Conclusions:

RcMYB21 specifically regulates flavonoid biosynthesis and accumulation in Rubus chingii.
RcMYB21 enhances salt stress tolerance, likely through flavonol accumulation.
This study provides insights into the molecular mechanisms of flavonoid regulation and highlights the dual role of RcMYB21 in secondary metabolism and abiotic stress.