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LncRNA MIAT Protects Against Sevoflurane-Induced Cognitive Dysfunction in Neonatal Rats via the miR-15b-5p/Ccnd1
Xuewen Lu1, Xinyi He2, Zhenbin Zhan3
1Anesthesiology Department, Zhongda Hospital (Jiangbei), Southeast University, Nanjing, Jiangsu, China.
Synapse (New York, N.Y.)
|June 30, 2026
Summary
Long non-coding RNA MIAT protects against sevoflurane-induced cognitive dysfunction in neonatal rats. This neuroprotective effect involves the lncRNA MIAT/miR-15b-5p/Ccnd1 pathway, offering a potential therapeutic target.
Area of Science:
- Neuroscience
- Molecular Biology
- Developmental Biology
Background:
- Sevoflurane (Sev) anesthesia can induce cognitive dysfunction in neonates.
- Long non-coding RNAs (lncRNAs) play crucial roles in neurological processes.
- Understanding the mechanisms of Sev-induced neurotoxicity is vital for clinical intervention.
Purpose of the Study:
- To investigate the role of lncRNA MIAT in sevoflurane-induced cognitive dysfunction in neonatal rats.
- To elucidate the underlying molecular mechanism involving miR-15b-5p and Ccnd1.
- To identify potential therapeutic targets for preventing sevoflurane-related neurotoxicity.
Main Methods:
- Sevoflurane-induced cognitive dysfunction model in 7-day-old Sprague-Dawley rats.
- Behavioral tests: Open Field Test (OFT), Novel Object Recognition (NOR), Morris Water Maze (MWM).
- Molecular analyses: RT-qPCR for lncRNA MIAT, miR-15b-5p, Ccnd1; dual-luciferase reporter assay for target validation.
Main Results:
- Sev exposure downregulated hippocampal lncRNA MIAT expression.
- lncRNA MIAT overexpression alleviated Sev-induced cognitive impairment in behavioral tests.
- lncRNA MIAT directly targeted miR-15b-5p, and miR-15b-5p targeted Ccnd1.
- The neuroprotective effect of lncRNA MIAT was mediated by sponging miR-15b-5p to upregulate Ccnd1.
Conclusions:
- lncRNA MIAT ameliorates sevoflurane-induced cognitive dysfunction in neonatal rats.
- The mechanism involves the lncRNA MIAT/miR-15b-5p/Ccnd1 axis.
- lncRNA MIAT represents a potential therapeutic target for preventing sevoflurane neurotoxicity.
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