Phospholipid metabolism in rapeseed roots orchestrates low-phosphorus-induced microbiome changes and interactions...

The role of microorganisms in enhancing plant phosphorus (P) acquisition is increasingly recognized, but little is known about the genetic factors that enable plants to shape a microbiome responsive to low-P conditions. In this study, we demonstrate that plant phospholipid metabolism influences low-P-induced changes in the root microbiome of rapeseed (Brassica napus). The bacterial genus Massilia was identified as a key biomarker whose abundance was significantly influenced by phospholipase-associated genetic factors. Experimental inoculation with Massilia significantly promoted plant growth and increased membrane phospholipid levels. Moreover, phospholipids produced by Massilia served as sources of bioavailable P for the host plant, while the plant-derived phospholipid metabolite glyceric acid served as a carbon source for the bacterium. These findings provide genetic insight into the mechanisms by which plant phospholipid metabolism, mediated by phospholipases, orchestrates the composition and activity of root microbial communities under low-P stress. This study also highlights the potential of Massilia as a bioinoculant for improving crop performance on P-deficient soils.