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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

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Molecular characterization of Staphylococcus aureus isolates from humans, cats, and dogs using the 16S rRNA gene

Karar Ali Abdulkhuder1, Mahmood Nabeel Awad2, Khetam Qaid Mayea3

  • 1Department of Medical Laboratory Technology, The Islamic University, Diwaniyah, Iraq.

Open Veterinary Journal
|June 30, 2026
PubMed
Abstract

Insights

Staphylococcus aureus exhibits genetic diversity across human, cat, and dog populations, suggesting potential zoonotic transmission. 16S rRNA gene sequencing aids in tracking this pathogen and assessing public health risks.

Area of Science:

  • Microbiology
  • Genetics
  • Veterinary Medicine

Background:

  • Staphylococcus aureus is a versatile pathogen causing infections in humans and animals.
  • Ocular infections are a concern in companion animals like cats and dogs.
  • 16S rRNA gene sequencing offers reliable bacterial identification and phylogenetic analysis.

Purpose of the Study:

  • To molecularly characterize Staphylococcus aureus isolates from humans, cats, and dogs.
  • To assess the genetic diversity of S. aureus across different hosts.
  • To evaluate the potential for zoonotic transmission between humans and companion animals.

Main Methods:

  • Ocular swab samples collected from 100 humans, 50 cats, and 50 dogs.
  • Bacterial identification via culture, biochemical tests, and PCR amplification of the 16S rRNA gene.
  • Phylogenetic analysis of 25 representative S. aureus isolates based on antibiotic resistance profiles.

Main Results:

  • 16S rRNA gene sequencing confirmed all isolates as Staphylococcus aureus.
  • Genetic analysis revealed similarities and variations among isolates from different hosts.
  • Evidence suggests possible cross-species transmission between humans and companion animals.

Conclusions:

  • Staphylococcus aureus isolates from humans, cats, and dogs display genetic diversity.
  • Companion animals may play a role in the zoonotic transmission of S. aureus.
  • 16S rRNA gene sequencing is crucial for monitoring bacterial evolution and public health risks.
Keywords:
16S rRNAGenetic diversityMolecular characterizationStaphylococcus aureusZoonotic transmission

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