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Published on: March 18, 2014
Bacteriological and histopathological study of Raoultella planticola in local rabbits with respiratory distress
Khudhair A Hussein1, Asmaa H Abdullah2, Alahmer M A3
1Department of Anatomy, Histology, University of Fallujah College of Medicine, Baghdad, Iraq.
Background:
The bacteriological and histopathological study of Raoultella planticola in local rabbits is not presented in previous research. There is no specific information about studying this bacterium in local rabbits.Hypothesis: The present study was based on bacteriological and histopathological changes in the trachea and lungs of local rabbits, as the samples were collected from 50 unhealthy local rabbits that appeared to be suffering from respiratory signs.
Aims:
To study the bacteria that were isolated for the first time from sheep suffering from respiratory infections, and diagnose them through VITEk2.
Methods:
We injected the isolated bacteria into (50) group of local rabbits (Oryctolagus cuniculus) and studied the histopathological changes in the trachea and lungs. Due to their similar morphological characteristics, our investigation reported this bacterium as Gram-negative rods. Then used the VITEK2 system to confirm the species of bacteria (93% Probability).
Results:
The animals clinically showed results (dullness with nasal and ocular discharge and cough). Then, euthanasia was performed to sacrifice animals seven days post-infection. The trachea and lungs were used in a histopathological study using routine Hematoxylin and Eosin stained. In this study, emphysema and bronchitis are often grouped under the heading of chronic obstructive pulmonary disease (COPD).
Conclusion:
Raoultella planticola infections are uncertain, and further studies are required. The previous difficulties in the identification of R. planticola may be explained by the introduction of more detailed identification techniques, which are explained in the present study. The conclusion of this study also provides that Raoultella planticola causes mild to moderate emphysema in local rabbits.

