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Related Experiment Video

Updated: Jul 1, 2026

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
08:26

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

Published on: April 9, 2013

Spectral Flow Cytometry Method for Immunophenotyping Neutrophil Activation and NETs in an Acute Dust Exposure Model.

Logan S Dean1,2, Maëlis J L Wahl3, Pinaki Mondal4

  • 1Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, Colorado, USA.

Immunity, Inflammation and Disease
|June 30, 2026
PubMed
Summary
This summary is machine-generated.

Acute organic dust extract (ODE) exposure alters neutrophil populations and increases neutrophil extracellular trap (NET) formation in the lungs. This study reveals dynamic neutrophil changes following inhalation injury.

Keywords:
NETacute lung injuryflow cytometryneutrophilsorganic dust exposure

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Published on: October 18, 2024

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Toxicology

Background:

  • Limited understanding of neutrophil dynamics during acute lung injury from dust inhalation.
  • Existing research on neutrophil response to acute exposures is constrained by blood and lung sample limitations.

Purpose of the Study:

  • To investigate neutrophil population dynamics and neutrophil extracellular trap (NET) formation following acute organic dust extract (ODE) exposure.
  • To characterize changes in neutrophil progenitors, banded, and mature neutrophils in bone marrow, blood, lung, and bronchoalveolar lavage fluid (BALF).

Main Methods:

  • Development of a spectral flow cytometry panel to identify various neutrophil populations.
  • Analysis of neutrophil surface markers (CD11b, CXCR2, CXCR4, CD62L) and cell characteristics (size, granularity).
  • Quantification of lytic and vital NET-forming neutrophils using Hoechst 33342 intensity.

Main Results:

  • Acute ODE exposure increased band and mature neutrophils in the lung and BALF, with a decrease in bone marrow and blood.
  • Pro-neutrophil proportions were altered in the blood post-ODE.
  • Neutrophils in the lung and BALF showed significant changes in CD11b, CXCR2, and CXCR4 expression, while CD62L was elevated in the blood.
  • Increased NET-forming neutrophil counts were observed in the lung and BALF following ODE exposure.

Conclusions:

  • Spectral flow cytometry effectively elucidates neutrophil dynamics, activation, and NET formation after acute ODE exposure.
  • Findings provide critical insights into the early neutrophil response to inhaled organic dust.