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Related Experiment Video

Updated: Jul 2, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

A Deep-Red Emissive Cage-in-Rings Complex for Lysosome Imaging.

Hui-Juan Wang1,2, Yutong Liu3,4, Yu Wang1

  • 1State Key Laboratory of Advanced Materials for Intelligent Sensing, Key Laboratory of Organic Integrated Circuit, Department of Chemistry, School of Science, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Tianjin University, Tianjin, China.

Angewandte Chemie (International Ed. in English)
|June 30, 2026
PubMed
Summary

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Researchers developed a novel cage-in-rings bioimaging probe (TPBCage6+⊂3CB[8]) for enhanced cellular imaging. This probe exhibits improved water solubility, biocompatibility, and deep-red emission, enabling selective lysosome visualization.

Area of Science:

  • Supramolecular Chemistry
  • Bioimaging Probes
  • Chemical Biology

Background:

  • Developing biocompatible, long-wavelength emissive, and selective bioprobes is crucial for bioimaging and clinical applications.
  • Existing bioimaging probes often face challenges with water solubility, non-specific interactions, and limited emission wavelengths.

Purpose of the Study:

  • To construct a novel cage-in-rings bioimaging probe with enhanced properties for subcellular imaging.
  • To investigate the supramolecular assembly of a hexacationic cage (TPBCage6+) with cucurbit[8]uril (CB[8]) and its impact on optical and biological characteristics.

Main Methods:

  • Stepwise assembly protocol utilizing noncovalent association between TPBCage6+ and CB[8].
  • Characterization of the resulting complex's conformation, cellular uptake, and photophysical properties (emission wavelength, quantum yield).
Keywords:
bioimaging technologycage‐in‐rings complexdeep‐red emissionlysosome imaging

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Last Updated: Jul 2, 2026

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  • Evaluation of biocompatibility and lysosome-selective imaging capabilities in vitro.

    • Main Results:

    • Successfully synthesized the TPBCage6+⊂3CB[8] complex with a C2-symmetrical conformation.
    • The complex demonstrated improved aqueous solubility, suppressed π-π stacking, and efficient cellular uptake.
    • Encapsulation by CB[8] induced a significant red shift in emission (552 nm to 652 nm) and enhanced fluorescence quantum yield.

    Conclusions:

    • The developed cage-in-rings supramolecular strategy enables precise control over bioimaging probe properties.
    • The TPBCage6+⊂3CB[8] probe facilitates effective lysosome-selective imaging in the deep-red region due to its enhanced characteristics.
    • This approach offers a new avenue for designing intrinsically selective and advanced bioimaging agents.