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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jul 1, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
11:46

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Published on: August 22, 2019

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Haozhe Wang1,2,3, Bowen Song4,5, Zhixing Wu1,3,6

  • 1Department of Biosciences and Bioinformatics, Center for Intelligent RNA Therapeutics, Suzhou Key Laboratory of Cancer Biology and Chronic Disease, School of Science, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China.

Nucleic Acids Research
|June 30, 2026
PubMed
Summary

VeloRM, a new computational tool, tracks RNA modifications in single cells. It distinguishes modifications before and after splicing, revealing new insights into RNA regulation during cell transitions.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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Related Experiment Videos

Last Updated: Jul 1, 2026

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
11:46

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Published on: August 22, 2019

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • Computational Biology
  • Genomics

Background:

  • RNA modifications are crucial for RNA function but their dynamics are poorly understood.
  • Cellular transitions involve complex regulatory changes, including in RNA modifications.

Purpose of the Study:

  • To develop a computational framework (VeloRM) for analyzing RNA modification dynamics at single-cell resolution.
  • To differentiate epitranscriptomes before and after RNA splicing.
  • To model epitranscriptomic changes and reconstruct cellular trajectories.

Main Methods:

  • Development of the VeloRM computational framework.
  • Analysis of single-cell RNA modification data (m6A and A-to-I editing).
  • Modeling of epitranscriptomic velocities to predict RNA modification states and cellular trajectories.

Main Results:

  • VeloRM successfully recapitulates known cell cycle and differentiation trajectories.
  • Identification of novel m6A modification sites on prespliced RNAs near splice junctions.
  • Demonstration of dynamic m6A patterns with functional implications in splicing regulation.

Conclusions:

  • VeloRM provides a powerful tool for studying epitranscriptome dynamics during biological transitions.
  • The framework enables unprecedented insights into RNA modification regulation.
  • New findings highlight the role of m6A modifications in splicing regulation.