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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jul 1, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
07:30

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Published on: May 10, 2018

Insulin Detection Enabled by Sensors Employing Fluorescence Signal Amplification Strategy.

Jiangnan Wang1, Tingting Feng1

  • 1College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, China.

Luminescence : the Journal of Biological and Chemical Luminescence
|June 30, 2026
PubMed
Summary

We developed a novel fluorescent aptamer sensor using single-walled carbon nanohorns for sensitive insulin detection. This method offers a simple, specific, and amplified fluorescence signal for accurate insulin quantification.

Keywords:
Cryonase enzymefluorescent sensorinsulinsingle‐walled carbon nanohorns

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Accurate insulin detection is crucial for diabetes management.
  • Existing methods for insulin detection can be complex or lack sensitivity.
  • Aptasensors offer a promising alternative due to their specificity and affinity.

Purpose of the Study:

  • To develop a novel fluorescent aptamer sensor for sensitive and specific insulin detection.
  • To utilize single-walled carbon nanohorns (SWCNHs) as a platform for enhanced fluorescence sensing.
  • To achieve signal amplification for improved detection limits.

Main Methods:

  • Development of a fluorescent aptamer sensor based on SWCNHs.
  • Utilizing fluorescence resonance energy transfer (FRET) for signal generation.
  • Employing aptamer-target binding and enzyme-catalyzed hydrolysis for signal amplification.

Main Results:

  • The sensor demonstrated high affinity and specificity for insulin.
  • A linear detection range from 5 to 250 ng/mL was achieved.
  • A low detection limit of 3.98 ng/mL was obtained with amplified fluorescence signals.

Conclusions:

  • The developed aptasensor provides a simple, sensitive, and specific method for insulin detection.
  • SWCNHs combined with aptamers and enzyme cycling offer a robust platform for biosensing.
  • This technology has potential applications in clinical diagnostics and diabetes monitoring.